{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T21:51:40Z","timestamp":1792705900991,"version":"4.5.0"},"reference-count":83,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T00:00:00Z","timestamp":1765843200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T00:00:00Z","timestamp":1765843200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100012025","name":"New York University Abu Dhabi","doi-asserted-by":"publisher","award":["AD266"],"award-info":[{"award-number":["AD266"]}],"id":[{"id":"10.13039\/100012025","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Tertiary lymphoid organs (TLOs) are non\u2010encapsulated immune structures that emerge in response to chronic inflammation, orchestrating local adaptive immune responses. However, recapitulating their complexity in vitro remains challenging due to the difficulty in generating\u00a0 physiologically relevant stromal\u2010immune interactions. Here, a 3D lymphoid tissue model is presented, engineered using human adipose\u2010derived stem cells (ADSCs) differentiated into fibroblastic reticular cell (FRC)\u2010like populations within collagen matrices. Differentiation is induced using TNF\u2010\u03b1 and LT\u2010\u03b1, with or without IL\u20104, generating two stromal phenotypes: FRC\n                    <jats:sub>P1<\/jats:sub>\n                    and FRC\n                    <jats:sub>P2<\/jats:sub>\n                    . These subsets exhibit matrix remodeling, distinct transcriptional signatures, and surface markers consistent with lymph node\u2010resident T cell reticular and follicular dendritic cell subsets. Upon co\u2010culture with peripheral blood mononuclear cells (PBMCs) and SARS\u2010CoV\u20102 S1\u2010primed mature dendritic cells, the model supports antigen\u2010specific B cell activation and cytokine environments indicative of Th1 or Th2 polarization. FRC\n                    <jats:sub>P1<\/jats:sub>\n                    favors B cell support and IgM secretion, whereas FRC\n                    <jats:sub>P2<\/jats:sub>\n                    promotes dendritic cell activation and Th1\u2010type chemokine expression. This platform demonstrates the functional diversification of stem cell\u2010derived FRC\u2010like subsets and their role in orchestrating immune microenvironments. It enables investigation of lymphoid tissue remodeling, stromal\u2010immune crosstalk, and antibody generation using total PBMCs, providing a scalable, customizable system for personalized vaccine screening, autoimmune modeling, and therapeutic development.\n                  <\/jats:p>","DOI":"10.1002\/advs.202513245","type":"journal-article","created":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T14:20:51Z","timestamp":1765894851000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Engineering Adaptive Immunity in 3D: A Patient\u2010Specific Lymphoid Model Using Stromal Networks and Peripheral Blood Mononuclear Cells"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1508-0560","authenticated-orcid":false,"given":"Mei","family":"ElGindi","sequence":"first","affiliation":[{"name":"Laboratory for Immuno Bioengineering Research and Applications Division of Engineering New 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