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Here, defined synthetic consortia are constructed by pairing\n                    <jats:italic>Shewanella oneidensis<\/jats:italic>\n                    MR\u20101, a model bidirectional electroactive bacterium, with\n                    <jats:italic>Clostridium aceticum<\/jats:italic>\n                    (electroactive acetogen) or\n                    <jats:italic>Acetobacterium woodii<\/jats:italic>\n                    (nonelectroactive acetogen), mimicking functional guilds commonly observed in natural MES communities. Co\u2010cultivation markedly boosts acetate production by up to 88%, achieving 1.16\u00a0\u00b1\u00a00.01 and 1.05\u00a0\u00b1\u00a00.01\u00a0g\u00a0L\n                    <jats:sup>\u22121<\/jats:sup>\n                    , with carbon conversion efficiencies exceeding 84%. Comprehensive electrochemical, spectroscopic, and biomass analyses reveal distinct spatial electron transfer modes: DIET via cytochrome\n                    <jats:italic>c<\/jats:italic>\n                    and riboflavin dominates at the biofilm\u2010electrode interface in\n                    <jats:italic>S. oneidensis\u2013C. aceticum<\/jats:italic>\n                    consortia, whereas\n                    <jats:italic>S. oneidensis\u2013A. woodii<\/jats:italic>\n                    consortia prefer H\n                    <jats:sub>2<\/jats:sub>\n                    \/formate\u2010mediated IET in the planktonic phase. This metabolic stratification enables\n                    <jats:italic>S. oneidensis<\/jats:italic>\n                    to function as an \u201cecosystem engineer,\u201d orchestrating electron flow to optimize CO\n                    <jats:sub>2<\/jats:sub>\n                    \u2010to\u2010acetate conversion across biofilm and suspension niches. The proposed synthetic ecology strategy provides a blueprint for designing high\u2010efficiency MES consortia, paving new avenues for sustainable carbon capture and bio\u2010based chemical production.\n                  <\/jats:p>","DOI":"10.1002\/advs.202513340","type":"journal-article","created":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T14:35:13Z","timestamp":1763130913000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Defined Electrosynthetic Microbial Consortia Reveal Electron Transfer Modes Governing Acetate Production"],"prefix":"10.1002","volume":"13","author":[{"given":"Jing","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Environment and Ecology Jiangnan University  Wuxi 214122 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9671-6457","authenticated-orcid":false,"given":"He","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Environment and Ecology Jiangnan University  Wuxi 214122 China"},{"name":"Jiangsu Collaborative Innovation Center of Water Treatment Technology and Material  Suzhou 215011 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qihao","family":"Cao","sequence":"additional","affiliation":[{"name":"School of Environment and Ecology Jiangnan University  Wuxi 214122 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Environment and Ecology Jiangnan University  Wuxi 214122 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Environment and Ecology Jiangnan University  Wuxi 214122 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minhua","family":"Cui","sequence":"additional","affiliation":[{"name":"School of Environment and Ecology Jiangnan University  Wuxi 214122 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Environment and Ecology Jiangnan University  Wuxi 214122 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Fu","sequence":"additional","affiliation":[{"name":"School of Environment and Ecology Jiangnan University  Wuxi 214122 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongbo","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Environment and Ecology Jiangnan University  Wuxi 214122 China"},{"name":"Jiangsu Collaborative Innovation Center of Water Treatment Technology and Material  Suzhou 215011 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,14]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2016.149"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-022-12031-9"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.2c04368"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2016.93"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-019-0173-x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00496-15"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02642-10"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00103-10"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rser.2015.03.003"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abb1214"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-021-00597-6"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01168-15"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jece.2025.118408"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41396-020-0627-1"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2025.132324"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2018.06.013"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.1841081"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.electacta.2019.03.085"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00449-016-1600-2"},{"key":"e_1_2_8_20_1","volume-title":"Electrochemical Methods: Fundamentals and Applications","author":"Bard A. 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