{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T18:29:21Z","timestamp":1773340161175,"version":"3.50.1"},"reference-count":48,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T00:00:00Z","timestamp":1766102400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T00:00:00Z","timestamp":1766102400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL171911"],"award-info":[{"award-number":["HL171911"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL158932"],"award-info":[{"award-number":["HL158932"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Vaccine\u2010induced immune thrombotic thrombocytopenia (VITT) is a new disorder that emerged in the wake of coronavirus disease 2019 (COVID\u201019) vaccination. It is a rare but life\u2010threatening condition that requires an aggressive course of treatment to improve patient outcomes. Currently, the diagnosis of VITT relies on a comprehensive panel of criteria, including a history of recent vaccination, platelet count, positive enzyme linked immunosorbent assays (ELISA)\u00a0result for a closely related thrombotic disorder, heparin\u2010induced thrombocytopenia (HIT), and platelet factor 4 (PF4)\u2010dependent functional assays. The study describes a technically simple antigenic assay designed to detect autoimmune antibodies associated with VITT. It is first shown that cross\u2010linked platelet factor 4 (c\u2010PF4) represents an antigenic target specific for VITT\/VITT\u2010like Abs. This antigenic target is then incorporated into a microfluidic electrochemical biosensor and specific and sensitive detection of VITT\/VITT\u2010like antibodies is demonstrated in a fully automated manner while using microliter volumes of patient sera. Fifty\u2010five serum samples are tested using the microfluidic electrochemical biosensor and demonstrated high accuracy for the detection of VITT sera and VITT\u2010like monoclonal gammopathy of thrombotic ignificance (MGTS)\u00a0antibodies compared to healthy controls and HIT patients. It is envisioned that the microfluidic electrochemical biosensor will be used in combination with other clinical criteria to enable timely, sensitive, and specific diagnosis of VITT.<\/jats:p>","DOI":"10.1002\/advs.202513607","type":"journal-article","created":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T17:39:42Z","timestamp":1766165982000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Microfluidic Electrochemical Biosensor for the Detection of Platelet Factor 4 Antibody\u2010Mediated Thrombotic Disorders"],"prefix":"10.1002","volume":"13","author":[{"given":"Diana F.","family":"Cedillo\u2010Alcantar","sequence":"first","affiliation":[{"name":"Department of Physiology and Biomedical Engineering Mayo Clinic Rochester MN 55902 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adam","family":"Kanack","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology Mayo Clinic Rochester MN 55902 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seonhwa","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biomedical Engineering Mayo Clinic Rochester MN 55902 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan M.","family":"Gonzalez\u2010Suarez","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biomedical Engineering Mayo Clinic Rochester MN 55902 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kihak","family":"Gwon","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biomedical Engineering Mayo Clinic Rochester MN 55902 USA"},{"name":"Department of Biofibers and Biomaterials Science Kyungpook National University Daegu 41566 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emily","family":"Mauch","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology Mayo Clinic Rochester MN 55902 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thi Thanh\u2010Qui","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biomedical Engineering Mayo Clinic Rochester MN 55902 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anand","family":"Padmanabhan","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology Mayo Clinic Rochester MN 55902 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8737-3250","authenticated-orcid":false,"given":"Alexander","family":"Revzin","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biomedical Engineering Mayo Clinic Rochester MN 55902 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,19]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2352-3026(21)00306-9"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2022017696"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.26542"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.26659"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2022007766"},{"key":"e_1_2_9_6_1","first-page":"73","volume":"140","author":"Kanack A. 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