{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T02:36:20Z","timestamp":1778639780561,"version":"3.51.4"},"reference-count":59,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T00:00:00Z","timestamp":1767916800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T00:00:00Z","timestamp":1767916800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["530287567"],"award-info":[{"award-number":["530287567"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["CRC1279"],"award-info":[{"award-number":["CRC1279"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013297","name":"Max-Planck-Institut f\u00fcr Polymerforschung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013297","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Broad\u2010spectrum antivirals are urgently needed to counter emerging viral threats. Targeting the viral envelope, an essential, conserved, and host\u2010derived structure, offers a promising strategy with a low risk of resistance. Here, we report the in silico design and experimental characterization of P1.6, a 24\u2010mer peptide generated using an evolutionary molecular dynamics (Evo\u2010MD) platform and optimized to sense and exploit lipid packing defects in viral membranes. Among nine Evo\u2010MD\u2013derived candidates, P1.6 showed the strongest membrane\u2010disruptive activity and inhibited HIV\u20101, Zika virus, and herpes simplex viruses with IC\n                    <jats:sub>50<\/jats:sub>\n                    values ranging from \u223c0.06 to 3.5\u00a0\u00b5\n                    <jats:sc>m<\/jats:sc>\n                    . P1.6 efficiently disrupted virus\u2010like liposomes without causing cytotoxicity or hemolysis at antiviral concentrations. All\u2010atom MD simulations predicted a predominantly \u03b1\u2010helical solution structure with a central kink and flexible termini. Upon membrane engagement, this kink was largely lost, yielding a more continuous and stabilized helix. ATR\u2010FTIR spectroscopy confirmed the membrane\u2010induced increase in helicity. Coarse\u2010grained MD simulations further demonstrated that P1.6 stabilizes transient membrane pores, while electron microscopy of treated HIV\u20101 particles revealed extensive envelope rupture and capsid release. Together, these results establish P1.6 as a potent membrane\u2010active antiviral lead and highlight the utility of Evo\u2010MD\u2013guided peptide design to target conserved biophysical vulnerabilities in viral envelopes.\n                  <\/jats:p>","DOI":"10.1002\/advs.202513911","type":"journal-article","created":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T12:25:32Z","timestamp":1767961532000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Identification and Characterization of an\n                    <i>In\u00a0Silico<\/i>\n                    Designed Membrane\u2010Active Peptide with Antiviral Properties"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0533-6999","authenticated-orcid":false,"given":"Pascal","family":"von Maltitz","sequence":"first","affiliation":[{"name":"Institute of Molecular Virology Ulm University Medical Center  Ulm 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Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4507-4882","authenticated-orcid":false,"given":"Jeroen","family":"Methorst","sequence":"additional","affiliation":[{"name":"Department of Physics Technical University Dortmund Dortmund Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-8889-0515","authenticated-orcid":false,"given":"Dennis","family":"Aschmann","sequence":"additional","affiliation":[{"name":"Leiden Institute of Chemistry Leiden University Leiden The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3983-3048","authenticated-orcid":false,"given":"Alexander","family":"Kros","sequence":"additional","affiliation":[{"name":"Leiden Institute of Chemistry Leiden University Leiden The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4632-2684","authenticated-orcid":false,"given":"Clarissa","family":"Read","sequence":"additional","affiliation":[{"name":"Central Facility for Electron Microscopy Ulm University  Ulm Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8875-3757","authenticated-orcid":false,"given":"Jasmina","family":"Ga\u010danin","sequence":"additional","affiliation":[{"name":"Max Planck Institute for Polymer Research  Mainz Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1410-6570","authenticated-orcid":false,"given":"Herre Jelger","family":"Risselada","sequence":"additional","affiliation":[{"name":"Department of Physics Technical University Dortmund Dortmund Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7316-7141","authenticated-orcid":false,"given":"Jan","family":"M\u00fcnch","sequence":"additional","affiliation":[{"name":"Institute of Molecular Virology Ulm University Medical Center  Ulm 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