{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T06:36:33Z","timestamp":1784097393518,"version":"3.55.0"},"reference-count":88,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T00:00:00Z","timestamp":1765497600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T00:00:00Z","timestamp":1765497600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21AG077631"],"award-info":[{"award-number":["R21AG077631"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R03NS123733"],"award-info":[{"award-number":["R03NS123733"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R03NS128459"],"award-info":[{"award-number":["R03NS128459"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21AG074978"],"award-info":[{"award-number":["R21AG074978"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012443","name":"Maryland Stem Cell Research Fund","doi-asserted-by":"publisher","award":["2022\u2010MSCRFD\u20105886"],"award-info":[{"award-number":["2022\u2010MSCRFD\u20105886"]}],"id":[{"id":"10.13039\/100012443","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012443","name":"Maryland Stem Cell Research Fund","doi-asserted-by":"publisher","award":["2022\u2010MSCRFL\u20105893"],"award-info":[{"award-number":["2022\u2010MSCRFL\u20105893"]}],"id":[{"id":"10.13039\/100012443","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Intracerebral cell transplantation holds promise for treating stroke and neurological disorders, yet challenges in precise delivery and post\u2010engraftment monitoring impede progress. This work introduces OPTRACE (OPtical imaging\u2010guided Transplantation and tRAcking of CElls), a two\u2010step optical framework integrating real\u2010time visualization during transplantation with longitudinal post\u2010transplantation in vivo cell tracking. Leveraging cost\u2010effective translucent glass micropipettes and two innovative predictive mathematical modeling\u2014the retention\u2013depth model (predicts retained fraction versus injection depth) and the hypoperfusion\u2013volume model (predicts hypoperfused fraction versus graft volume)\u2014that this work fits to data (depth\u2013retention R\n                    <jats:sup>2<\/jats:sup>\n                    = 0.91; volume\u2013growth R\n                    <jats:sup>2<\/jats:sup>\n                    = 0.78)\u2014OPTRACE optimizes delivery parameters to maximize engraftment and minimize hypoxia. A novel pulse\u2010elevation injection technique further enhances the precision of superficial cortical retention. Following transplantation, multicolor labeling combined with two\u2010photon fluorescence microscopy permits longitudinal single\u2010cell tracking, revealing host microglial responses, and altered neuronal calcium signaling at the graft interface. OPTRACE provides micrometer precision, longitudinal dynamics and quantitative insights of cells during and after transplantation, accelerating mechanistic understanding and therapeutic development for regenerative cell therapies.\n                  <\/jats:p>","DOI":"10.1002\/advs.202514183","type":"journal-article","created":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T04:49:21Z","timestamp":1765601361000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["OPTRACE: Optical Imaging\u2013Guided Transplantation and Tracking of Cells in the Mouse Brain"],"prefix":"10.1002","volume":"13","author":[{"given":"Jinghui","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Diagnostic Radiology and Nuclear Medicine University of Maryland School of Medicine Baltimore MD 21201 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honglin","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Radiology and Nuclear Medicine University of Maryland School of Medicine Baltimore MD 21201 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colleen","family":"Russell","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Radiology and Nuclear Medicine University of Maryland School of Medicine Baltimore MD 21201 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikolaj","family":"Walczak","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Radiology and Nuclear Medicine University of Maryland School of Medicine Baltimore MD 21201 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dawei","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Radiology and Nuclear Medicine University of Maryland School of Medicine Baltimore MD 21201 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanda","family":"Qiao","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Radiology and Nuclear Medicine University of Maryland School of Medicine Baltimore MD 21201 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxuan","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology University of Maryland School of Medicine Baltimore MD 21201 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengyan","family":"Chu","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Radiology and Nuclear Medicine University of Maryland School of Medicine Baltimore MD 21201 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miroslaw","family":"Janowski","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Radiology and Nuclear Medicine University of Maryland School of Medicine Baltimore MD 21201 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piotr","family":"Walczak","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Radiology and Nuclear Medicine University of Maryland School of Medicine Baltimore MD 21201 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4798-4882","authenticated-orcid":false,"given":"Yajie","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Radiology and Nuclear Medicine University of Maryland School of Medicine Baltimore MD 21201 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,12]]},"reference":[{"key":"e_1_2_10_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2017.03.003"},{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2018.05.004"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9112517"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-020-01762-z"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12975-022-00984-y"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/nep3.27"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2024.05.009"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/nep3.70003"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41536-017-0028-x"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/jphp.12362"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.119.026318"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp-2019-322515"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2023.11.001"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-27568-x"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3171\/2009.10.JNS09252"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.350193"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2009.04.097"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.2967\/jnumed.109.068304"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep13628"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11886-009-0073-1"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.15283\/ijsc23045"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.21037\/sci.2017.03.10"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2021.641970"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.111.637884"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.17.1.016004"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt899"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-024-06057-0"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth818"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/nep3.20"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/vsu.12639"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.3727\/096368916X692618"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.126268"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41536-017-0028-x"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.3727\/096368910X503406"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.3171\/jns.2004.101.2.0314"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.3389\/fradi.2024.1085834"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2021.102126"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12026-012-8349-8"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2024.11.036"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01433.2006"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(98)01200-1"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.3389\/fams.2023.1257066"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40635-024-00691-y"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.03.095"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2016.55"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2020.109042"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-020-0760-9"},{"key":"e_1_2_10_48_1","first-page":"4259","volume":"31","author":"Liang Y.","year":"1991","journal-title":"Cereb. Cortex (New York, NY)"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-40444-1"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1523\/ENEURO.0255-19.2019"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.40805"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.114.172510"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2017.09.007"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2338"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2017.03.039"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12975-017-0593-8"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot080143"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.3727\/096368911X566190"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.15923"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2009.0423"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41536-021-00134-2"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0093735"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06293"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12354"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.3727\/096368914X679354"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep33080"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2018.04.043"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.1113\/EP092219"},{"key":"e_1_2_10_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(17)30046-7"},{"key":"e_1_2_10_70_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-022-03736-8"},{"key":"e_1_2_10_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2013.04.001"},{"key":"e_1_2_10_72_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature20113"},{"key":"e_1_2_10_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2019.10.002"},{"key":"e_1_2_10_74_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.4127"},{"key":"e_1_2_10_75_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-05277-w"},{"key":"e_1_2_10_76_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-35536-3"},{"key":"e_1_2_10_77_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.53205"},{"key":"e_1_2_10_78_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.59244"},{"key":"e_1_2_10_79_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1902366116"},{"key":"e_1_2_10_80_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2023.1146031"},{"key":"e_1_2_10_81_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11060-016-2062-8"},{"key":"e_1_2_10_82_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2024.07.036"},{"key":"e_1_2_10_83_1","doi-asserted-by":"publisher","DOI":"10.1002\/med.21400"},{"key":"e_1_2_10_84_1","doi-asserted-by":"publisher","DOI":"10.3171\/FOC\/2008\/24\/3-4\/E14"},{"key":"e_1_2_10_85_1","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.119.025436"},{"key":"e_1_2_10_86_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1065-6995(03)00165-3"},{"key":"e_1_2_10_87_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_2_10_88_1","first-page":"1","volume":"4","author":"\u00d8yvind H.","year":"2001","journal-title":"Palaeontol. Electronica"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202514183","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/advs.202514183","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202514183","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T08:43:35Z","timestamp":1773305015000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202514183"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,12]]},"references-count":88,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1002\/advs.202514183"],"URL":"https:\/\/doi.org\/10.1002\/advs.202514183","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,12]]},"assertion":[{"value":"2025-07-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e14183"}}