{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T08:42:50Z","timestamp":1775983370982,"version":"3.50.1"},"reference-count":52,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,11,16]],"date-time":"2025-11-16T00:00:00Z","timestamp":1763251200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,16]],"date-time":"2025-11-16T00:00:00Z","timestamp":1763251200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Traditionally considered a sterile environment, the articular cavity has this perception overturned with advances in multi\u2010omics technologies\u2014microbial communities are increasingly identified in once\u2010thought sterile tissues, making the articular cavity a potential microbial niche. However, the presence and role of intra\u2010articular microbes in osteoarthritis (OA) remain rarely studied. Synovial fluid microbiomes of OA patients at different stages are analyzed via 16S rRNA high\u2010throughput sequencing, and the results show that microbial diversity is positively correlated with OA progression, with the microbiomes dominated by Proteobacteria, Firmicutes, and Actinobacteria. Culturing yields 145 strains, with\n                    <jats:italic>Micrococcus luteus (M. luteus)<\/jats:italic>\n                    \u00a0significantly enriched in advanced OA. In OA rats, intra\u2010articular transplantation of synovial microbes or\n                    <jats:italic>M. luteus<\/jats:italic>\n                    G18 exacerbates cartilage damage. Mechanistically,\n                    <jats:italic>M. luteus<\/jats:italic>\n                    G18 activates the TLR2\/JNK\/AP\u20101 pathway via surface peptidoglycan, disrupting chondrocyte homeostasis to inhibit extracellular matrix (ECM) synthesis and promote degradation. These findings not only provide the first comprehensive evidence of the joint cavity microbiota but also unveil\n                    <jats:italic>M. luteus<\/jats:italic>\n                    G18 as a microbial driver of OA progression. This study reshapes the understanding of OA pathogenesis and opens new avenues for microbial\u2010based diagnostics and therapeutics\u2014pointing toward a previously overlooked dimension of joint biology that deserves further exploration.\n                  <\/jats:p>","DOI":"10.1002\/advs.202514220","type":"journal-article","created":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T07:32:01Z","timestamp":1763364721000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Synovial Fluid\u2010derived\n                    <i>Micrococcus Luteus<\/i>\n                    G18 Exacerbates Osteoarthritis Progression by Promoting Chondrocyte Degradation via TLR2\/JNK\/AP\u20101 Signaling Pathway"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0506-8536","authenticated-orcid":false,"given":"Tingtao","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Orthopedics The Second Affiliated Hospital Jiangxi Medical College Nanchang University  Nanchang Jiangxi 330006 China"},{"name":"Jiangxi Province Key Laboratory of Bioengineering Drugs School of Pharmacy Jiangxi Medical College Nanchang University  Nanchang Jiangxi 330031 China"},{"name":"National Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Jiangxi Medical College Nanchang University  Nanchang 330031 China"},{"name":"School of Life Sciences Nanchang University  Nanchang Jiangxi 330031 China"},{"name":"College of Food Science &amp; Technology Nanchang University  Nanchang Jiangxi 330031 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingwei","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The Second Affiliated Hospital Jiangxi Medical College Nanchang University  Nanchang Jiangxi 330006 China"},{"name":"Jiangxi Province Key Laboratory of Bioengineering Drugs School of Pharmacy Jiangxi Medical College Nanchang University  Nanchang Jiangxi 330031 China"},{"name":"National Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Jiangxi Medical College Nanchang University  Nanchang 330031 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tangchang","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Life Sciences Nanchang University  Nanchang Jiangxi 330031 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinyue","family":"Qi","sequence":"additional","affiliation":[{"name":"School of Life Sciences Nanchang University  Nanchang Jiangxi 330031 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaiyi","family":"Li","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Jiangxi Medical College Nanchang University  Nanchang 330031 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Wei","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Jiangxi Medical College Nanchang University  Nanchang 330031 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-7855-3308","authenticated-orcid":false,"given":"Liang","family":"Hao","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The Second Affiliated Hospital Jiangxi Medical College Nanchang University  Nanchang Jiangxi 330006 China"},{"name":"Institute of Orthopedics of Jiangxi Province  Nanchang Jiangxi 330006 China"},{"name":"Institute of Minimally Invasive Orthopedics Nanchang University  Nanchang Jiangxi 330006 China"},{"name":"Jiangxi Provincial Key Laboratory of Spine and Spinal Cord Disease  Nanchang Jiangxi 330006 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,16]]},"reference":[{"key":"e_1_2_10_1_1","doi-asserted-by":"publisher","DOI":"10.1530\/EOR-23-0191"},{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2020.22171"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2665-9913(23)00163-7"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.mm7241a1"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcp1903768"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-34830-4"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2023.101864"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41413-023-00304-6"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.39304"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2016.136"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-023-01330-w"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/8293921"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2020.11102"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jse.2018.04.019"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard-2022-222871"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202406027"},{"key":"e_1_2_10_17_1","volume":"12","author":"Hawkins M. R.","year":"2025","journal-title":"Open Forum Infect. Dis."},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-025-02517-9"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.8b08785"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2014.09.019"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12272-011-0113-4"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(14)60802-3"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21072358"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41584-025-01223-y"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arth.2023.03.084"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.22.00594"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-32675-w"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21228618"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12017-025-08883-9"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.32604\/biocell.2025.066108"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13258-023-01471-w"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/cosmetics9060121"},{"key":"e_1_2_10_33_1","first-page":"218","volume":"22","author":"Kocur M.","year":"1972","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.33073\/pjm-2021-030"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-50-1-4"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-07-2319"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.3389\/fped.2023.1303040"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.idcr.2019.e00657"},{"key":"e_1_2_10_39_1","first-page":"43","volume":"11","author":"Pederson A.","year":"2023","journal-title":"Case Rep."},{"key":"e_1_2_10_40_1","first-page":"1","volume":"2","author":"Mahmood A. A.","year":"2021","journal-title":"J. Zankoy Sulaimani"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.9b04477"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2023.102080"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2025.108525"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmrt.2023.03.130"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2021.03.002"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm14041263"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2024.03.004"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2024.117897"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI12466"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.09.075"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-024-01995-7"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20225724"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202514220","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/advs.202514220","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202514220","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T07:47:58Z","timestamp":1775980078000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202514220"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,16]]},"references-count":52,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1002\/advs.202514220"],"URL":"https:\/\/doi.org\/10.1002\/advs.202514220","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,16]]},"assertion":[{"value":"2025-07-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e14220"}}