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This study establishes an electric field\u2010induced directional migration model using fluorescence tension probes to visualize microfilament forces and intracellular osmotic pressure dynamics. Electric fields induce protein kinase A (PKA)\u2010dependent PDLIM7 recruitment, polarizing microfilament tension at cell edges via site\u2010specific phosphorylation at serine 190. Concurrently, the electric field induces asymmetric changes in membrane potential, driven by protein nanoparticles and calcium ions, regulating osmotic tension. Leading\u2010edge depolarization activates TMEM16A channel, while trailing\u2010edge hyperpolarization activates the small conductance calcium\u2010activated potassium (SK) channel. Chloride influx and potassium efflux create differential ion diffusion, resulting in leading\u2010edge hypertonic expansion and trailing\u2010edge contraction, thereby dictating the directionality of electrotactic migration. 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