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Here, we introduce the Multimodal Imaging Platform for Organoid Tracking (MIPOT), integrating MRI, bioluminescence imaging, surgical microscopy, and intravital two\u2010photon fluorescence microscopy to monitor CO transplantation in chronic stroke lesions in mice. MIPOT enabled confirmation of initial graft placement within cleaned infarct cavities, longitudinal assessment of viability\u2010associated bioluminescence signals, and high\u2010resolution visualization of graft\u2010derived cells and short\u2010term cellular dynamics. Bioluminescence imaging revealed a progressive decline in graft\u2010associated signals that stabilized at approximately 25% by two weeks, while endpoint histology confirmed limited persistence of human graft\u2010derived cells at later time points. 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