{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T16:25:24Z","timestamp":1790439924366,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T00:00:00Z","timestamp":1768176000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T00:00:00Z","timestamp":1768176000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Antimicrobial peptides (AMPs) are promising alternatives to overcome antimicrobial resistance (AMR). However, precise construction of an AMP targeting bacterial cell membranes derived from natural peptides remains a great challenges. Although the artificial intelligence (AI) algorithm\u2010assisted screening method has achieved unprecedented successes, it's difficult to predict the targets of AMPs obtained from this method. To address this, an AMP (\n                    <jats:bold>P 3\u20103R\u20108I<\/jats:bold>\n                    ) based on several natural peptides derived from insect cuticle was constructed precisely via amino acid mutation. The mutated amino acids Arginine (R) and Isoleucine (I) are expected to target the bacterial cell membranes. Surprisingly,\n                    <jats:bold>P 3\u20103R\u20108I<\/jats:bold>\n                    exhibits super antibacterial capability against two representative bacteria: methicillin\u2010resistant\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    (MRSA) and\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    (\n                    <jats:italic>E. coli<\/jats:italic>\n                    ), which could be attributed to the ability to quickly penetrate bacterial cell membranes and then to bind to bacterial DNA of\n                    <jats:bold>P 3\u20103R\u20108I<\/jats:bold>\n                    , resulting in the suppression of DNA replication. In rats\u2019 model, the MRSA\u2010infected wound could be alleviated by\n                    <jats:bold>P 3\u20103R\u20108I<\/jats:bold>\n                    obviously, as well as lung and spleen infections in MRSA\u2010induced systemic sepsis. Our findings provide a prospect for the precise construction of AMPs targeting bacterial cell membranes as well as a means of overcoming AMR, offering a strategy for drug\u2010resistant bacteria\u2010induced tissue repair.\n                  <\/jats:p>","DOI":"10.1002\/advs.202517068","type":"journal-article","created":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T08:27:39Z","timestamp":1768292859000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Precise Construction of an Antimicrobial Peptide Targeting Bacterial Cell Membranes Derived From Natural Peptides"],"prefix":"10.1002","volume":"13","author":[{"given":"Jiaqi","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Thoracic Surgery The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an P. R. China"},{"name":"Key Laboratory of Enhanced Recovery After Surgery of Integrated Chinese and Western Medicine Administration of Traditional Chinese Medicine of Shaanxi Province The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bohao","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an P. R. China"},{"name":"Key Laboratory of Enhanced Recovery After Surgery of Integrated Chinese and Western Medicine Administration of Traditional Chinese Medicine of Shaanxi Province The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingzhuo","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an P. R. 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinjuan","family":"Huang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Porous Metal Materials Shaanxi International Research Center for Soft Matter School of Materials Science and Engineering Xi'an Jiaotong University  Xi'an P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jizhao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an P. R. China"},{"name":"Key Laboratory of Enhanced Recovery After Surgery of Integrated Chinese and Western Medicine Administration of Traditional Chinese Medicine of Shaanxi Province The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangjian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an P. R. China"},{"name":"Key Laboratory of Enhanced Recovery After Surgery of Integrated Chinese and Western Medicine Administration of Traditional Chinese Medicine of Shaanxi Province The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7924-8890","authenticated-orcid":false,"given":"Qiuyu","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an P. R. 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