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Multimodal neural platforms that integrate optical and electrical modalities offer a promising approach that surpasses resolution limits. Over the last decade, transparent graphene microelectrodes have been proposed as highly suitable multimodal neural interfaces. However, their fabrication commonly relies on the manual transfer process of pre\u2010grown graphene sheets which introduces reliability and scalability issues. In this study, multilayer graphene microelectrode arrays (MEAs) with electrode sizes as small as 10\u201350\u00a0\u00b5m in diameter, are fabricated using a transfer\u2010free process on a transparent substrate for in vitro multimodal platforms. For the first time, the capability of transparent graphene electrodes with a diameter of just 10\u00a0\u00b5m to reliably capture extracellular spiking activity with high signal\u2010to\u2010noise ratios (up to \u223c25\u00a0dB) is demonstrated. The recorded signal quality is found to be more limited by the electrode\u2010tissue coupling than the MEA technology itself. Overall, this study shows the potential of transfer\u2010free multilayer graphene MEAs to interface with neural tissue, paving the way to advancing neuroscientific research through the next\u2010generation of multimodal neural interfaces.<\/jats:p>","DOI":"10.1002\/advs.202517524","type":"journal-article","created":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T10:00:16Z","timestamp":1770199216000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Transparent Transfer\u2010Free Ultrasmall Multilayer Graphene Microelectrodes Enable High Quality Recordings in Brain Slices"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-9031-3388","authenticated-orcid":false,"given":"Nerea","family":"de Alvarez de Eulate","sequence":"first","affiliation":[{"name":"Department of Microelectronics Faculty of Electrical Engineering Mathematics and Computer Science Delft University of Technology  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