{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T13:09:45Z","timestamp":1792069785886,"version":"4.4.0"},"reference-count":36,"publisher":"Wiley","issue":"28","license":[{"start":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T00:00:00Z","timestamp":1773273600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T00:00:00Z","timestamp":1773273600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT, South Korea","doi-asserted-by":"publisher","award":["RS\u20102025\u201000553834"],"award-info":[{"award-number":["RS\u20102025\u201000553834"]}],"id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT, South Korea","doi-asserted-by":"publisher","award":["RS\u20102025\u201023963632"],"award-info":[{"award-number":["RS\u20102025\u201023963632"]}],"id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Thioamides constitute an important class of pharmaceutically active natural products, yet their discovery and development are limited. A targeted metabologenomic method is developed to logically and efficiently discover thioamide compounds in bacteria. To this end, two strains were identified to possess genetic capacity for biosynthesizing thioamides from the bacterial genomic DNA library (1,192 strains) using the polymerase chain reaction to target the TfuA\u2010encoding gene, a genomic hallmark of thioamide biosynthesis. Mass spectrometric isotopic patterns of sulfur\u2010bearing compounds serve as metabolomic hallmarks to detect thioamide production from the extracts of the selected strains without chromatography. Applying this metabologenomic targeting approach, two new thioamides, thiogochangamides A and B, belonging to the thioviridamide family whose stereochemistry has remained unresolved for two decades, were discovered in\n                    <jats:italic>Streptomyces<\/jats:italic>\n                    sp. Their absolute configurations were fully assigned through chemical derivatizations, including the advanced Marfey method, Mosher method, partial hydrolysis, synthesis of an unusual amino acid, and desulfurization, combined with computational methods. Thiogochangamide B exhibits potent inhibitory activity against gemcitabine\u2010resistant pancreatic cancer cells both in vitro and in vivo. Mechanistically, thiogochangamide B effectively downregulates Wnt\/\u03b2\u2010catenin signaling, thereby suppressing the metastatic potential of drug\u2010resistant cancer cells. This study provides a new therapeutic strategy for overcoming recalcitrant drug\u2010resistant pancreatic cancer.\n                  <\/jats:p>","DOI":"10.1002\/advs.202517849","type":"journal-article","created":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T11:05:18Z","timestamp":1773313518000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Metabologenomic Hallmark\u2010Based Discovery of Bacterial Thioamides as a New Lead against Drug\u2010Resistant Pancreatic Cancer"],"prefix":"10.1002","volume":"13","author":[{"given":"Young Eun","family":"Du","sequence":"first","affiliation":[{"name":"Natural Products Research Institute and Research Institute of Pharmaceutical Sciences, College of Pharmacy Seoul National University Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun Seo","family":"Bae","sequence":"additional","affiliation":[{"name":"Natural Products Research Institute and Research Institute of Pharmaceutical Sciences, College of Pharmacy Seoul National University Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thinh T. M.","family":"Bui","sequence":"additional","affiliation":[{"name":"College of Pharmacy Kyung Hee University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seok Beom","family":"Lee","sequence":"additional","affiliation":[{"name":"Natural Products Research Institute and Research Institute of Pharmaceutical Sciences, College of Pharmacy Seoul National University Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dahan","family":"Kim","sequence":"additional","affiliation":[{"name":"Research Institute of Pharmaceutical Sciences College of Pharmacy Seoul National University Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin\u2010Gyeong","family":"Park","sequence":"additional","affiliation":[{"name":"Research Institute of Pharmaceutical Sciences College of Pharmacy Seoul National University Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongjoo","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Chemistry Seoul National University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soo Yeon","family":"Park","sequence":"additional","affiliation":[{"name":"Natural Products Research Institute and Research Institute of Pharmaceutical Sciences, College of Pharmacy Seoul National University Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sangwook","family":"Kang","sequence":"additional","affiliation":[{"name":"Natural Products Research Institute and Research Institute of Pharmaceutical Sciences, College of Pharmacy Seoul National University Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beomsu","family":"Lee","sequence":"additional","affiliation":[{"name":"Natural Products Research Institute and Research 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