{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T17:03:14Z","timestamp":1784307794295,"version":"3.55.0"},"reference-count":41,"publisher":"Wiley","issue":"26","license":[{"start":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T00:00:00Z","timestamp":1772582400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T00:00:00Z","timestamp":1772582400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["2020JCPT02"],"award-info":[{"award-number":["2020JCPT02"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFC3405100"],"award-info":[{"award-number":["2023YFC3405100"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2024M762000"],"award-info":[{"award-number":["2024M762000"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The spatiotemporal dynamics of protein kinase diffusion govern signal activation cascades, thereby modulating fundamental cellular functions. Pseudokinases, catalytically inactive members of the protein kinase superfamily, utilize noncatalytic signaling mechanisms to exert pivotal cellular functions and are frequently dysregulated in human diseases. While nanoscale dynamics of catalytically active receptors regulate signaling integrity, the functional significance of pseudokinase spatial organization remains unknown. Here, using aptamer\u2010based single\u2010molecule tracking in living cells, we observed heterogeneous diffusion modes of pseudokinase PTK7 (confined, Brownian, and directed motion). Specifically, spatially PTK7 diffusion coefficients (D) quantitatively correlate with metastatic potential across pancreatic, colorectal and breast cancer cell lines. Functional validation demonstrates that antibody\u2010mediated PTK7 immobilization suppresses invasion, while Epithelial\u2010Mesenchymal Transition (EMT) induction accelerates diffusion kinetics to promote metastasis. Crucially, faster PTK7 mobility increases stochastic collision frequency with tyrosine kinase\u2010like orphan receptor 2 (ROR2), enhancing complex formation to robustly activate the WNT\/PCP pathway. Moreover, in patient\u2010derived primary cells, accelerated PTK7 kinetics positively correlate with invasive and metastatic phenotypes, confirming the clinical relevance of this biophysical regulatory mechanism. This work establishes pseudokinase spatial dynamics as a biophysical regulator of tumor progression, revealing a non\u2010catalytic paradigm where receptor diffusion kinetics encode cellular behavior through stochastic signaling potentiation.<\/jats:p>","DOI":"10.1002\/advs.202517876","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T17:02:10Z","timestamp":1772989330000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Spatiotemporal Organization of PTK7 Diffusion on Cell Surface Facilitates Tumor Invasion and Migration"],"prefix":"10.1002","volume":"13","author":[{"given":"Yaohua","family":"Li","sequence":"first","affiliation":[{"name":"Institute of Molecular Medicine (IMM) Renji Hospital School of Medicine Shanghai Jiao Tong University Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery Renji Hospital School of Medicine Shanghai Jiao Tong University Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Systems Medicine For Cancer of Oncology Department and Shanghai Cancer Renji Hospital School of Medicine Shanghai Jiao Tong University Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sainan","family":"Guo","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine (IMM) Renji Hospital School of Medicine Shanghai Jiao Tong University Shanghai P. R. 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Sun","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine (IMM) Renji Hospital School of Medicine Shanghai Jiao Tong University Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8066-1524","authenticated-orcid":false,"given":"Weihong","family":"Tan","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine (IMM) Renji Hospital School of Medicine Shanghai Jiao Tong University Shanghai P. R. China"},{"name":"Zhejiang Cancer Hospital Hangzhou Institute of Medicine (HIM) Chinese Academy of Sciences  Hangzhou P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2559"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573\u2010019\u20100018\u20103"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41388\u2010024\u201003060\u2010x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.108187"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature24264"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1181729"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3277"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.04.018"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2018.02.034"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202303390"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202004764"},{"key":"e_1_2_8_13_1","first-page":"2179","article-title":"Colon\u2010carcinoma 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