{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T21:00:46Z","timestamp":1791406846189,"version":"4.3.3"},"reference-count":36,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81572818"],"award-info":[{"award-number":["81572818"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Cell therapy and oncolytic viruses have emerged as promising cancer treatments but face significant challenges in solid tumors due to immune suppression and gene\u2010related toxicities. Here, we selected a probiotic\n                    <jats:italic>Lactobacillus rhamnosus<\/jats:italic>\n                    (LR) that appears to exert oncolytic activity by inducing massive calcium influx, which subsequently triggers a lethal ROS burst in tumor cells. To reduce systemic toxicity and enhance oncolytic efficacy at the tumor site, we designed molecular pili (MP) targeting collagen\u2010rich solid tumors and modified them into LRs via chemical coupling (LR@MP). In mouse models of colorectal cancer and melanoma, LR@MP increased intratumoral accumulation by two times and enhanced bacterial clearance from peripheral tissues. At a safe dose of 4 \u00d7 10\n                    <jats:sup>5<\/jats:sup>\n                    CFU, LR@MP inhibited 60%\u201380% of tumor growth. This dual\u2010optimization strategy provides a new approach for next\u2010generation in vivo therapies and warrants further preclinical evaluation.\n                  <\/jats:p>","DOI":"10.1002\/advs.202517989","type":"journal-article","created":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T15:53:56Z","timestamp":1770134036000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Oncolytic Probiotics with Molecular Pili for Solid Tumor Therapy"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-0115-7052","authenticated-orcid":false,"given":"Haodong","family":"Ge","sequence":"first","affiliation":[{"name":"Department of General Surgery Ruijin Hospital Shanghai Jiao Tong University School of Medicine  Shanghai P. R. China"},{"name":"Department of General Surgery, Shanghai Key Laboratory of Gastric Neoplasms, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengsheng","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of General Surgery Ruijin Hospital Shanghai Jiao Tong University School of Medicine  Shanghai P. R. China"},{"name":"Department of General Surgery, Shanghai Key Laboratory of Gastric Neoplasms, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of General Surgery Ruijin Hospital Shanghai Jiao Tong University School of Medicine  Shanghai P. R. China"},{"name":"Department of General Surgery, Shanghai Key Laboratory of Gastric Neoplasms, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Si","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Biliary\u2010Pancreatic Surgery Renji Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changjie","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine (IMM) State Key Laboratory of Oncogenes and Related Genes Shanghai Cancer Institute Department of Oncology Renji Hospital School of Medicine Shanghai Jiao Tong University  Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuchen","family":"Hou","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery The First Affiliated Hospital of USTC Division of Life Sciences and Medicine University of Science and Technology of China  Hefei P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongye","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanghai Cancer Institute State Key Laboratory of Systems Medicine for Cancer Renji Hospital Shanghai Jiao Tong University School of Medicine  Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linke","family":"Bian","sequence":"additional","affiliation":[{"name":"Shanghai Cancer Institute State Key Laboratory of Systems Medicine for Cancer Renji Hospital Shanghai Jiao Tong University School of Medicine  Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Zhong","sequence":"additional","affiliation":[{"name":"Department of General Surgery Ruijin Hospital Shanghai Jiao Tong University School of Medicine  Shanghai P. R. China"},{"name":"Department of General Surgery, Shanghai Key Laboratory of Gastric Neoplasms, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifan","family":"Qu","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases Shanghai Institute of Traumatology and Orthopaedics Ruijin Hospital Shanghai Jiao Tong University School of Medicine  Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luyang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of General Surgery Ruijin Hospital Shanghai Jiao Tong University School of Medicine  Shanghai P. R. China"},{"name":"Department of General Surgery, Shanghai Key Laboratory of Gastric Neoplasms, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junjun","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of General Surgery Ruijin Hospital Shanghai Jiao Tong University School of Medicine  Shanghai P. R. China"},{"name":"Department of General Surgery, Shanghai Key Laboratory of Gastric Neoplasms, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengwei","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases Shanghai Institute of Traumatology and Orthopaedics Ruijin Hospital Shanghai Jiao Tong University School of Medicine  Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6938-9582","authenticated-orcid":false,"given":"Wenguo","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases Shanghai Institute of Traumatology and Orthopaedics Ruijin Hospital Shanghai Jiao Tong University School of Medicine  Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minhua","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of General Surgery Ruijin Hospital Shanghai Jiao Tong University School of Medicine  Shanghai P. R. China"},{"name":"Department of General Surgery, Shanghai Key Laboratory of Gastric Neoplasms, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1707447"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2014.58.3377"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2934"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006692"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/8451728"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2002.20.1.142"},{"key":"e_1_2_8_8_1","first-page":"1691","article-title":"Safety Assessment of Probiotics for Human Use","volume":"59","author":"Sanders M. E.","year":"2010","journal-title":"Gut Microbes"},{"key":"e_1_2_8_9_1","first-page":"159","article-title":"Insight from 100 years of Research with Probiotic E Coli","volume":"5","author":"Wassenaar T. 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