{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T22:00:15Z","timestamp":1790373615741,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T00:00:00Z","timestamp":1768953600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T00:00:00Z","timestamp":1768953600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100013076","name":"National Major Science and Technology Projects of China","doi-asserted-by":"publisher","award":["2025ZD0551300"],"award-info":[{"award-number":["2025ZD0551300"]}],"id":[{"id":"10.13039\/501100013076","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013076","name":"National Major Science and Technology Projects of China","doi-asserted-by":"publisher","award":["2025ZD0551301"],"award-info":[{"award-number":["2025ZD0551301"]}],"id":[{"id":"10.13039\/501100013076","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82125022"],"award-info":[{"award-number":["82125022"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82241059"],"award-info":[{"award-number":["82241059"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82202633"],"award-info":[{"award-number":["82202633"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005230","name":"Natural Science Foundation of Chongqing Municipality","doi-asserted-by":"publisher","award":["CSTB2022NSCQ\u2010MSX1357"],"award-info":[{"award-number":["CSTB2022NSCQ\u2010MSX1357"]}],"id":[{"id":"10.13039\/501100005230","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002865","name":"Chongqing Municipal Science and Technology Bureau","doi-asserted-by":"publisher","award":["2025ZYZD003"],"award-info":[{"award-number":["2025ZYZD003"]}],"id":[{"id":"10.13039\/501100002865","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018531","name":"Major Science and Technology Projects in Yunnan Province","doi-asserted-by":"publisher","award":["202302AA310039"],"award-info":[{"award-number":["202302AA310039"]}],"id":[{"id":"10.13039\/501100018531","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Precise imaging of cancer cells\u00a0serves as the foundation for subtype analysis, significantly advancing the development of precision medicine. The unavoidable cellular internalization of fluorescent labels constrains the resolution and timeliness, presenting a significant obstacle. This study introduces a dynamic decorating strategy of DNA scaffolds that enables the execution of four distinct Boolean logic operations through self\u2010assembly and self\u2010disassembly. By integrating the in\u2010built molecular circuit, the proposed assay achieved signal\u2010amplified detection of low\u2010abundance nucleic acid inputs. In the application of cell imaging, inputs were labeled with aptamers to operate membrane\u2010confined self\u2010assembly or self\u2010disassembly of DNA scaffolds on the cell surface, enabling simultaneous identification of distinct subtypes of cancer cells with high fidelity. The intrinsic durability of DNA scaffolds successfully prevented cellular internalization for up to 300 min, boosting the long\u2010duration imaging. Moreover, the assay was capable of profiling a broad spectrum of cancer cell abundances from as low as 0.1% to 10% in clinical blood samples, consistently achieving recognition efficiency exceeding 60%. These findings underscore the transformational potential of DNA scaffold\u2010based imaging tools in biological research and precision medicine.<\/jats:p>","DOI":"10.1002\/advs.202518307","type":"journal-article","created":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T06:24:35Z","timestamp":1769063075000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Dynamic Decoration of DNA Scaffolds for High\u2010Resolution Cancer Cell Subtyping"],"prefix":"10.1002","volume":"13","author":[{"given":"Xiaolin","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine Chongqing Center For Clinical Laboratory School of Medicine Chongqing Academy of Medical Sciences Chongqing General Hospital Chongqing University  Chongqing P. R. China"},{"name":"College of Bioengineering Key Laboratory For Biorheological Science and Technology of Ministry of Education Chongqing University  Chongqing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Chongqing Center For Clinical Laboratory School of Medicine Chongqing Academy of Medical Sciences Chongqing General Hospital Chongqing University  Chongqing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinlin","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Chongqing Center For Clinical Laboratory School of Medicine Chongqing Academy of Medical Sciences Chongqing General Hospital Chongqing University  Chongqing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liangting","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Pathology and Southwest Cancer Center Southwest Hospital Third Military Medical University (Army Medical University) and Key Laboratory of Tumor Immunopathology Ministry of Education of China  Chongqing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengheng","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Chongqing Center For Clinical Laboratory School of Medicine Chongqing Academy of Medical Sciences Chongqing General Hospital Chongqing University  Chongqing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaopei","family":"Qiu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Chongqing Center For Clinical Laboratory School of Medicine Chongqing Academy of Medical Sciences Chongqing General Hospital Chongqing University  Chongqing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heng","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Chongqing Center For Clinical Laboratory School of Medicine Chongqing Academy of Medical Sciences Chongqing General Hospital Chongqing University  Chongqing P. R. China"},{"name":"College of Bioengineering Key Laboratory For Biorheological Science and Technology of Ministry of Education Chongqing University  Chongqing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Chongqing Center For Clinical Laboratory School of Medicine Chongqing Academy of Medical Sciences Chongqing General Hospital Chongqing University  Chongqing P. R. China"},{"name":"College of Bioengineering Key Laboratory For Biorheological Science and Technology of Ministry of Education Chongqing University  Chongqing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxing","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Science and Laboratory Medicine Kunming Medical University  Kunming Yunnan P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxuan","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Chongqing Center For Clinical Laboratory School of Medicine Chongqing Academy of Medical Sciences Chongqing General Hospital Chongqing University  Chongqing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junsong","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Bioengineering Key Laboratory For Biorheological Science and Technology of Ministry of Education Chongqing University  Chongqing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Chongqing Center For Clinical Laboratory School of Medicine Chongqing Academy of Medical Sciences Chongqing General Hospital Chongqing University  Chongqing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Bernardini","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine University of Rome Tor Vergata  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaoyong","family":"Yang","sequence":"additional","affiliation":[{"name":"The State Key Laboratory of Physical Chemistry of Solid Surfaces MOE Key Laboratory of Spectrochemical Analysis &amp; Instrumentation Key Laboratory for Chemical Biology of Fujian Province Department of Chemical Biology College of Chemistry and Chemical Engineering Xiamen University  Xiamen P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Chongqing Center For Clinical Laboratory School of Medicine Chongqing Academy of Medical Sciences Chongqing General Hospital Chongqing University  Chongqing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0166-9027","authenticated-orcid":false,"given":"Yang","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Chongqing Center For Clinical Laboratory School of Medicine Chongqing Academy of Medical Sciences Chongqing General Hospital Chongqing University  Chongqing P. R. China"},{"name":"College of Life Science and Laboratory Medicine Kunming Medical University  Kunming Yunnan P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,21]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.04.018"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2748"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.3226"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010018\u20100519\u2010y"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj\u20102023\u2010074962"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571\u2010024\u201000883\u20101"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010022\u201001339\u20106"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2023.02.045"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201054438\u20100"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202103993"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202301083"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202406330"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010023\u201037752\u2010x"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.2c20024"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.3c11208"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202011198"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202309837"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja509263k"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.8b04319"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.9b05470"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nantod.2023.101817"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202410642"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.1c02738"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.2c13735"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scib.2024.10.032"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.2c01382"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202300614"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41565\u2010023\u201001468\u20102"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010023\u201006890\u2010z"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201046339\u2010z"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41557\u2010019\u20100251\u20108"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41557\u2010022\u201000945\u2010w"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/nn502058j"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.8b00660"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596\u2010024\u201001050\u20107"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202505714"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2022.114932"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2024.156270"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201052986\u2010z"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.2c12360"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.2c02456"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/smtd.202400917"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202509727"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2200121"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/355564a0"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0602615103"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202518307","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/advs.202518307","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202518307","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T21:22:59Z","timestamp":1790371379000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202518307"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,21]]},"references-count":46,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1002\/advs.202518307"],"URL":"https:\/\/doi.org\/10.1002\/advs.202518307","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,21]]},"assertion":[{"value":"2025-09-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e18307"}}