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Recently developed Chimeric Antigen Receptor (CAR) \u2010T cell therapy has shown potent clinical efficacy against hematological malignancies. However, solid tumors, including ovarian cancer, possess several mechanisms that hinder T cell activity, and metabolic alteration of cancer cells has been shown to contribute to resistance to immune cell attack against solid tumors. Here, we explore the metabolic response of ovarian cancer cells to CAR\u2010T cell attack using label\u2010free high\u2010content hyperspectral stimulated Raman scattering (h\n                    <jats:sup>2<\/jats:sup>\n                    SRS) imaging. Utilizing visible h\n                    <jats:sup>2<\/jats:sup>\n                    SRS imaging with much improved spatial resolution, we find an altered cholesterol metabolism, featured by increased storage of cholesteryl ester in lipid droplets and free cholesterol, in ovarian cancer cells that survive the CAR\u2010T treatment. Administration of Avasimibe, an inhibitor of cholesteryl esterification, further enhances CAR\u2010T cytotoxicity. Our study shows the promise of implementing metabolic modulation to facilitate CAR\u2010T cell treatment of solid tumors.\n                  <\/jats:p>","DOI":"10.1002\/advs.202518334","type":"journal-article","created":{"date-parts":[[2026,2,15]],"date-time":"2026-02-15T11:05:15Z","timestamp":1771153515000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["High\u2010Content SRS Imaging Unveils Altered Cholesterol Metabolism in Ovarian Cancers Under CAR\u2010T Treatment"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9568-7770","authenticated-orcid":false,"given":"Chinmayee V.","family":"Prabhu Dessai","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering Boston University  Boston Massachusetts USA"},{"name":"Photonics Center Boston University  Boston Massachusetts 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