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During metastatic progression, distant organs form a pre\u2010metastatic niche (PMN), creating a permissive microenvironment that facilitates circulating tumor cell (CTC) colonization. To investigate pulmonary PMN formation in breast cancer, a micro\u2010organ chip is employed that enables contact\u2010independent coculture of tumor and lung tissues. This model reveals that PMN formation is governed by tumor\u2010secreted factors without requiring direct tumor cell contact and exhibits non\u2010tumor\u2010type specificity. It is found that coculture with tumor tissue upregulates vascular endothelial growth factor (VEGF) receptor\u20102 (VEGFR2\/KDR) in lung capillary cells. 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