{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T03:15:34Z","timestamp":1788405334983,"version":"build-2803163510"},"reference-count":72,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["1944167"],"award-info":[{"award-number":["1944167"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000025","name":"National Institute of Mental Health","doi-asserted-by":"publisher","award":["R01MH136852"],"award-info":[{"award-number":["R01MH136852"]}],"id":[{"id":"10.13039\/100000025","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R21 AI167709\u201001A1"],"award-info":[{"award-number":["R21 AI167709\u201001A1"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI186609"],"award-info":[{"award-number":["R01AI186609"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    HIV remains a major global public health challenge, causing 42.3 million deaths since its discovery in the early 1980s. Despite progress in prevention and treatment, around 60% of people with HIV (PWH) remain undiagnosed in resource\u2010limited regions due to the lack of inexpensive and equipment\u2010free detection methods. Here, we developed a low\u2010cost, robust, and label\u2010free CRISPR\u2010based diagnostic platform for detecting HIV viral load with minimal instrumentation. Our strategy combines rolling circle amplification (RCA) with plasmid reporter\u2010based ratiometric CRISPR (rCRISPR) that enables the detection of HIV RNA down to single\u2010digit aM sensitivity from PWH\u2010derived HIV samples ex vivo. Unlike conventional RCA, which requires fragmentations of long RNA target sequences, our design harnesses the triple functions of the phi29 DNA polymerase (namely exonuclease activity, polymerization, and strand displacement), enabling the detection of the long HIV genome without pre\u2010fragmentation. Cas12a reaction then detected RCA products by converting supercoiled \u03a6X174 plasmid reporters to relaxed forms. The target concentration was quantified based on the supercoil\u2010to\u2010relaxed plasmid ratio. Further, we constructed an all\u2010in\u2010one smartphone\u2010based minigel electrophoresis device to demonstrate equipment\u2010free HIV viral load testing. Finally, the assay has demonstrated for\n                    <jats:italic>BRAF<\/jats:italic>\n                    point mutation detection, showcasing the robustness of our strategy for broad disease diagnostic applications.\n                  <\/jats:p>","DOI":"10.1002\/advs.202519508","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T02:51:16Z","timestamp":1788403876000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Attomolar Detection of HIV\u20101 With Label\u2010Free RCA\u2010rCRISPR on Smartphone"],"prefix":"10.1002","author":[{"given":"Noor","family":"Mohammad","sequence":"first","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering North Carolina State University  Raleigh North Carolina USA"},{"name":"Department of Chemical Engineering Bangladesh University of Engineering and Technology  Dhaka Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasiia","family":"Steksova","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering North Carolina State University  Raleigh North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuyang","family":"Tang","sequence":"additional","affiliation":[{"name":"UNC HIV Cure Center and Department of Biochemistry &amp; Biophysics University of North Carolina  Chapel Hill North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leyao","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering North Carolina State University  Raleigh North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alireza","family":"Velayati","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering North Carolina State University  Raleigh North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengwei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering North Carolina State University  Raleigh North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aditi","family":"Dey Poonam","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering North Carolina State University  Raleigh North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sina","family":"Jamalzadegan","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering North Carolina State University  Raleigh North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Breen","sequence":"additional","affiliation":[{"name":"Department of Molecular Biomedical Sciences College of Veterinary Medicine North Carolina State University  Raleigh North Carolina USA"},{"name":"Comparative Medicine Institute North Carolina State University  Raleigh North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongjie","family":"Chen","sequence":"additional","affiliation":[{"name":"UNC HIV Cure Center and Department of Biochemistry &amp; Biophysics University of North Carolina  Chapel Hill North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahsa","family":"Bagi","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering North Carolina State University  Raleigh North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guochun","family":"Jiang","sequence":"additional","affiliation":[{"name":"UNC HIV Cure Center and Department of Biochemistry &amp; Biophysics University of North Carolina  Chapel Hill North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1214-010X","authenticated-orcid":false,"given":"Qingshan","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering North Carolina State University  Raleigh North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2025.110445"},{"key":"e_1_2_8_3_1","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1089\/aid.2013.0274","article-title":"Toward an Endgame: Finding and Engaging People Unaware of Their HIV\u20101 Infection in Treatment and Prevention","volume":"30","author":"Burns D. N.","year":"2014","journal-title":"Research and Human Retroviruses"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00064\u201018"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1231143"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/d41586\u2010020\u201002765\u20109"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3680"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature.2016.20988"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aar6245"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aam9321"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551\u2010021\u201000760\u20107"},{"key":"e_1_2_8_12_1","doi-asserted-by":"crossref","DOI":"10.1002\/anie.202214987","volume":"62","author":"Weng Z.","year":"2023","journal-title":"Angewandte Chemie International 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M.","year":"2018","journal-title":"JMIR Public Health Surveill"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202519508","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/advs.202519508","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202519508","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T02:51:26Z","timestamp":1788403886000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202519508"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,2]]},"references-count":72,"alternative-id":["10.1002\/advs.202519508"],"URL":"https:\/\/doi.org\/10.1002\/advs.202519508","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,2]]},"assertion":[{"value":"2025-10-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e19508"}}