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Therefore, a biphasic hydrogel model system is designed that recapitulates structural, biophysical, and biochemical components of the bone microenvironment to replicate early metastasis events. Breast cancer cells embedded within a glycosaminoglycan\u2010based nanoporous hydrogel phase are traced as they colonize a directly adjacent macroporous cryogel compartment, precisely and selectively equipped with specific bone\u2010like biomolecular signals and\/or solution\u2010deposited mineral crystals. Microscopic monitoring of the spatiotemporal cancer cell distributions yields colonization profiles that display the correlated effects of cell invasion, matrix interaction, and proliferation. MDA\u2010MB\u2010231 cells, but not MCF\u20107 cells, rapidly infiltrate the cryogel compartment at rates depending on the cross\u2010linking degree of the hydrogel phase. Cryogel functionalization with adhesion\u2010mediating peptide ligands enhances matrix interactions and survival\/proliferation of the MDA\u2010MB\u2010231 cells. When combined with cryogel\u2010released stromal cell\u2010derived factor 1 (SDF\u20101), survival\/proliferation are further amplified and additionally MDA\u2010MB\u2010231 cell invasion is promoted. The presence of deposited bone\u2010like mineral strongly impedes these responses and is accompanied by characteristic alterations in distinct cellular gene\u2010expression programs. The reported methodology may not only provide further mechanistic insights into early bone metastasis, but also facilitate the screening of anti\u2010metastatic drugs.<\/jats:p>","DOI":"10.1002\/advs.202519798","type":"journal-article","created":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T12:13:51Z","timestamp":1769516031000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Mineralized Cryogel\/Hydrogel Constructs to Recapitulate Early Breast Cancer Bone Metastasis In Vitro"],"prefix":"10.1002","volume":"13","author":[{"given":"Jana","family":"Sievers\u2010Liebschner","sequence":"first","affiliation":[{"name":"Division Polymer Biomaterials Science, Max Bergmann Center of Biomaterials Dresden Leibniz\u2010Institut f\u00fcr Polymerforschung Dresden e.V.  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