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Here, donor\u2010derived circulating podocytes (CPCs) are identified as a blood\u2010based surrogate of acute rejection. A magnetically reversible herringbone microfluidic chip equipped with epithelial cell adhesion molecule (EpCAM) antibody\u2010functionalized magnetic beads efficiently isolates CPCs from 1\u00a0mL of peripheral blood (capture efficiency 84.4%, release 95.5%, viability 96.0%). Among 65 participants, only transplant recipients with biopsy\u2010confirmed rejection exhibited\u226535 CPCs mL\n                    <jats:sup>\u2212<\/jats:sup>\n                    <jats:sup>1<\/jats:sup>\n                    (AUC = 1.0). Fluorescence in situ hybridization (FISH) of sex\u2010mismatched transplants confirmed donor origin. Single\u2010cell RNA\u2010seq of 10 isolated CPCs revealed up\u2010regulation of podocyte\u2010injury and innate\/adaptive immune genes (such as NF\u2010\u03baB, TNF, NOD\u2010like receptor pathways) in rejection versus stable samples. CPC enumeration thus provides a minimally invasive, mechanistically informed warning of renal allograft rejection.\n                  <\/jats:p>","DOI":"10.1002\/advs.202520426","type":"journal-article","created":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T19:29:32Z","timestamp":1773862172000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["RENAL\u2010CHIP: Rejection Evaluation via Non\u2010Invasive Analysis of Circulating Podocytes With Herringbone\u2010Chip Isolation Platform"],"prefix":"10.1002","volume":"13","author":[{"given":"Juan","family":"Song","sequence":"first","affiliation":[{"name":"Discipline of Intelligent Instrument and Equipment College of Chemistry and Chemical Engineering Department of Urology Xiang'an Hospital of Xiamen University School of Medicine, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM) Xiamen University  Xiamen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yilong","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology College of Life Sciences Fujian Agriculture and Forestry University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeyuan","family":"Zheng","sequence":"additional","affiliation":[{"name":"Discipline of Intelligent Instrument and Equipment College of Chemistry and Chemical Engineering Department of Urology Xiang'an Hospital of Xiamen University School of Medicine, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM) Xiamen University  Xiamen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Zhu","sequence":"additional","affiliation":[{"name":"Discipline of Intelligent Instrument and 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University School of Medicine, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM) Xiamen University  Xiamen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Zheng","sequence":"additional","affiliation":[{"name":"Discipline of Intelligent Instrument and Equipment College of Chemistry and Chemical Engineering Department of Urology Xiang'an Hospital of Xiamen University School of Medicine, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM) Xiamen University  Xiamen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiyuan","family":"Yu","sequence":"additional","affiliation":[{"name":"Discipline of Intelligent Instrument and Equipment College of Chemistry and Chemical Engineering Department of Urology Xiang'an Hospital of Xiamen University School of Medicine, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM) Xiamen University  Xiamen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianzhong","family":"Zheng","sequence":"additional","affiliation":[{"name":"Discipline of Intelligent Instrument and Equipment College of Chemistry and Chemical Engineering Department of Urology Xiang'an Hospital of Xiamen University School of Medicine, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM) Xiamen University  Xiamen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Shao","sequence":"additional","affiliation":[{"name":"Discipline of Intelligent Instrument and Equipment College of Chemistry and Chemical Engineering Department of Urology Xiang'an Hospital of Xiamen University School of Medicine, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM) Xiamen University  Xiamen 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