{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T22:04:35Z","timestamp":1790805875285,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T00:00:00Z","timestamp":1769558400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T00:00:00Z","timestamp":1769558400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82070719"],"award-info":[{"award-number":["82070719"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82572388"],"award-info":[{"award-number":["82572388"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82204091"],"award-info":[{"award-number":["82204091"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2024A1515030231"],"award-info":[{"award-number":["2024A1515030231"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010256","name":"Guangzhou Municipal Science and Technology Project","doi-asserted-by":"publisher","award":["2025A03J4319"],"award-info":[{"award-number":["2025A03J4319"]}],"id":[{"id":"10.13039\/501100010256","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010256","name":"Guangzhou Municipal Science and Technology Project","doi-asserted-by":"publisher","award":["2025A03J3082"],"award-info":[{"award-number":["2025A03J3082"]}],"id":[{"id":"10.13039\/501100010256","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Multidrug\u2010resistant urinary tract infections (MDR\u2010UTIs) remain one of the most challenging clinical infections globally owing to the paucity of effective therapeutic agents. Nanozymes\u2010triggered cuproptosis\u2010like bacterial death has emerged as a promising bactericidal strategy. However, its efficacy is compromised by insufficient catalytic sites and suboptimal synergy among active centers. Consequently, the development of nanozymes capable of efficiently inducing cuproptosis\u2010like death holds considerable potential for treating MDR\u2010UTIs. Herein, the Cu\u2010ZIF8\u2010Ru nanozyme was engineered that leverages an asymmetric heterobimetallic Cu\u2500N\u2500Ru bridged catalytic center to achieve efficient multienzyme activities and trigger cuproptosis\u2010like death in MDR\u2010UTIs. Theoretical simulations revealed that introducing Ru sites as secondary catalytic centers upshifts the Cu d\u2010band center, thereby lowering activation barriers and enhancing catalytic activity at the Cu\u2500N\u2500Ru active site. Cu\u2010ZIF8\u2010Ru nanozyme demonstrated strong bactericidal effects against MDR\u2010bacteria by promoting intracellular Cu\n                    <jats:sup>2<\/jats:sup>\n                    <jats:sup>+<\/jats:sup>\n                    accumulation and depleting cellular energy, ultimately leading to cuproptosis\u2010like death. A thermogel encapsulating Cu\u2010ZIF8\u2010Ru enabled intravesical in situ gelation and sustained release, providing durable bactericidal and anti\u2010inflammatory effects in both acute and recurrent MDR\u2010UTIs models with a favorable biosafety. In summary, Cu\u2010ZIF8\u2010Ru offers an efficient, controllable, and low\u2010toxicity intravesical bactericidal strategy that addresses key clinical demands in MDR\u2010UTIs management and holds substantial translational promise.\n                  <\/jats:p>","DOI":"10.1002\/advs.202520705","type":"journal-article","created":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T20:14:11Z","timestamp":1769631251000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Asymmetric Cu\u2500N\u2500Ru Bridgedsite Nanozyme\u2010Loaded Injectable\u00a0Thermogel Boosts Cuproptosis\u2010Like Death for Multidrug\u2010Resistant Urinary Tract Infections"],"prefix":"10.1002","volume":"13","author":[{"given":"Guanlin","family":"Li","sequence":"first","affiliation":[{"name":"Department of Urology the Second Affiliated Hospital of Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shike","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Urology the Second Affiliated Hospital of Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinggong","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Traditional Chinese Medicine Syndrome Orthopedics Department The Second Affiliated Hospital of Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Urology Guangdong Provincial Key Laboratory of Urological Diseases Guangdong Engineering Research Center of Urinary Minimally Invasive Surgery The First Affiliated Hospital Robot and Intelligent Equipment Guangzhou Institute of Urology Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Urology Guangdong Provincial Key Laboratory of Urological Diseases Guangdong Engineering Research Center of Urinary Minimally Invasive Surgery The First Affiliated Hospital Robot and Intelligent Equipment Guangzhou Institute of Urology Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongcan","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Urology the Second Affiliated Hospital of Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhao","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Urology the Second Affiliated Hospital of Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Public Health Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0723-5529","authenticated-orcid":false,"given":"Hongxing","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Urology Guangdong Provincial Key Laboratory of Urological Diseases Guangdong Engineering Research Center of Urinary Minimally Invasive Surgery The First Affiliated Hospital Robot and Intelligent Equipment Guangzhou Institute of Urology Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenqi","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Urology the Second Affiliated Hospital of Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,28]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579\u2010024\u201001092\u20104"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2016.09.003"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40779\u2010024\u201000569\u2010w"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591\u2010019\u20100503\u20106"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564\u2010022\u201001107\u2010x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202406843"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2025.106459"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/INMD.20220011"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1039\/D5CS00083A"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202506119"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.4c02825"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.4c06565"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201053410\u20102"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2024.216205"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202507939"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201045619\u2010y"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.3c12783"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202500601"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2025.123566"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.34133\/bmr.0014"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.4c09695"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202303432"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202307695"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.22203\/eCM.v045a10"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202308197"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202308850"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41565\u2010024\u201001618\u20100"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s44222\u2010024\u201000205\u20101"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202008720"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202408580"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202306376"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202419673"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201053488\u20108"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202506110"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2022.214760"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202307798"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202505123"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/EXP.20230113"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2024.135149"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2024.07.009"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12274\u2010024\u20106699\u2010x"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010025\u201061519\u20101"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202401252"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201053824\u2010y"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202312308"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202520705","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/advs.202520705","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202520705","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T10:17:49Z","timestamp":1775125069000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202520705"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,28]]},"references-count":45,"journal-issue":{"issue":"19","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1002\/advs.202520705"],"URL":"https:\/\/doi.org\/10.1002\/advs.202520705","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,28]]},"assertion":[{"value":"2025-10-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e20705"}}