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This metastability is also reflected in their sensitivity to environmental conditions and in intrinsic lattice dynamics, where spontaneous tubulin loss is balanced by tubulin incorporation from solution\u2014a process that also enables microtubules to self\u2010repair when damaged. Whether such intrinsic self\u2010repair is sufficient to preserve microtubule integrity during dynamic molecular motor\u2010induced buckling, which frequently occurs in cells, remains unclear. Here, we show that kinesin\u2010driven microtubule buckling in vitro induces severe lattice damage, leading to extensive tubulin incorporation. In many cases, however, the damage exceeds the microtubules\u2019 capacity for self\u2010repair, resulting in breakage. In contrast, microtubules survive continuous buckling substantially longer in the presence of intracellular factors. Our results identify the limits of intrinsic microtubule self\u2010repair and demonstrate that additional cellular mechanisms are essential to maintain microtubule integrity under sustained mechanical load.<\/jats:p>","DOI":"10.1002\/advs.202521721","type":"journal-article","created":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T10:54:18Z","timestamp":1773312858000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Kinesin\u2010Induced Buckling Reveals the Limits of Microtubule Self\u2010Repair"],"prefix":"10.1002","volume":"13","author":[{"given":"Shweta","family":"Nandakumar","sequence":"first","affiliation":[{"name":"Experimental Physics and Center for Biophysics Saarland University  Saarbr\u00fccken Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonas","family":"Bosche","sequence":"additional","affiliation":[{"name":"Theoretical Physics and Center for Biophysics Saarland University 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