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Despite their physiological relevance, the predictive power of organoids remains limited by the immature state of the constituent cells, posing a major challenge for mechanistic studies of adult physiology and late\u2010onset disorders. Here, we establish a strategy for enhancing the maturation status of human neuromuscular organoids (NMOs) through chronic Electrical Pulse Stimulation (EPS). We demonstrate that low\u2010frequency EPS, applied during the early stages of NMO development and maintained over several weeks, enhances neuromuscular maturation and functional output. Independent of stimulation waveform dynamics, EPS\u2010trained NMOs (EPS\u2010NMOs) display stronger and more frequent spontaneous contractions that persist long after stimulation has ceased. Quantitative imaging and transcriptomic analyses reveal a robust improvement in EPS\u2010NMO skeletal muscle and neural tissue morphology, coordinated regulation of lineage\u2010specific biomarkers, and upregulation of gene programs associated with neuromuscular maturation. Mechanobiological measurements further demonstrate increased tissue stiffness and faster relaxation dynamics in EPS\u2010NMOs, consistent with enhanced excitation\u2010contraction coupling (ECC) and force generation. Collectively, these findings establish EPS as a powerful, non\u2010invasive, and on\u2010demand modality for promoting the morphological and functional maturation of complex organoid systems.<\/jats:p>","DOI":"10.1002\/advs.202522762","type":"journal-article","created":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T10:08:13Z","timestamp":1782122893000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Enhancing Maturation of Human Neuromuscular Organoids via Electrical Stimulation"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9809-2764","authenticated-orcid":false,"given":"Chrysanthi\u2010Maria","family":"Moysidou","sequence":"first","affiliation":[{"name":"Max Delbr\u00fcck Center for Molecular Medicine (MDC)  Berlin 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