{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T22:34:04Z","timestamp":1792190044889,"version":"4.4.0"},"reference-count":68,"publisher":"Wiley","issue":"34","license":[{"start":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T00:00:00Z","timestamp":1775692800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T00:00:00Z","timestamp":1775692800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002701","name":"Ministry of Education","doi-asserted-by":"publisher","award":["RS\u20102025\u201002402969"],"award-info":[{"award-number":["RS\u20102025\u201002402969"]}],"id":[{"id":"10.13039\/501100002701","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["RS\u20102025\u201018362970"],"award-info":[{"award-number":["RS\u20102025\u201018362970"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["RS\u20102024\u201000392705"],"award-info":[{"award-number":["RS\u20102024\u201000392705"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003710","name":"Korea Health Industry Development Institute","doi-asserted-by":"publisher","award":["RS\u20102025\u201002223587"],"award-info":[{"award-number":["RS\u20102025\u201002223587"]}],"id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003625","name":"Ministry of Health and Welfare","doi-asserted-by":"publisher","award":["RS\u20102025\u201002223587"],"award-info":[{"award-number":["RS\u20102025\u201002223587"]}],"id":[{"id":"10.13039\/501100003625","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT, South Korea","doi-asserted-by":"publisher","award":["RS\u20102025\u201018362970"],"award-info":[{"award-number":["RS\u20102025\u201018362970"]}],"id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003716","name":"Korea Basic Science Institute","doi-asserted-by":"publisher","award":["RS\u20102025\u201002402969"],"award-info":[{"award-number":["RS\u20102025\u201002402969"]}],"id":[{"id":"10.13039\/501100003716","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Although mRNA vaccines have revolutionized modern vaccinology, significant challenges persist owing to their dependence on ultra\u2010cold storage, patient discomfort, and logistical complications associated with intramuscular (IM) injections. Dissolving microneedles (DMNs) are a promising alternative by enabling minimally invasive transdermal administration that directly engages skin\u2010resident immune cells, while their fabrication compromises fragile mRNA. Moreover, previous studies have emphasized antibody\u2010mediated immunity rather than exploiting their capacity for strong T cell responses. In this study, we developed an mRNA\u2010liposome vaccine via DMN (ML\u2010DMN) to preserve the mRNA activity in DMN formulations and boost immune cell activation. Incorporating excipients into the formulation retained the mRNA integrity during fabrication, as demonstrated by in vitro luciferase assays. In vivo administration of ovalbumin (OVA) mRNA via ML\u2010DMN promoted rapid migration and activation of antigen\u2010presenting cells (APCs) in draining lymph nodes (dLNs) within 24\u00a0h, initiating an innate immune response. This led to robust OVA\u2010specific T cell activation, proliferation, and secretion of key cytokines, confirming a potent adaptive immune response with lower mRNA doses than IM injections. These results suggest that ML\u2010DMN systems have significant potential to overcome current mRNA vaccine limitations by improving mRNA integration and immune cell targeting, offering a viable strategy for future vaccine development.<\/jats:p>","DOI":"10.1002\/advs.202522846","type":"journal-article","created":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T19:22:24Z","timestamp":1775762544000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Transdermal Delivery of an mRNA\u2010Liposome Vaccine via Dissolving Microneedle to Preserve Vaccine Activity and Enhance Immune Activation"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1162-6685","authenticated-orcid":false,"given":"Jeehye","family":"Nam","sequence":"first","affiliation":[{"name":"Department of Biotechnology Yonsei University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JiWon","family":"Ahn","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Yonsei University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiwoo","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Yonsei University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nahong","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Yonsei University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youjin","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Integrative Biotechnology Yonsei University  Inchon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung min","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Integrative Biotechnology Yonsei University  Inchon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Kim","sequence":"additional","affiliation":[{"name":"JUVIC Inc.  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geonwoo","family":"Kang","sequence":"additional","affiliation":[{"name":"JUVIC Inc.  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang\u2010Jun","family":"Ha","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Yonsei University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1946-7300","authenticated-orcid":false,"given":"Hyungil","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Yonsei University  Seoul Republic of Korea"},{"name":"JUVIC Inc.  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd.2017.243"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2021.718753"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010023\u201001579\u20101"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12272\u2010022\u201001381\u20107"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd4278"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2021.03.038"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010022\u201001294\u20102"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2117128"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40265\u2010021\u201001480\u20107"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20171450"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.1029069"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.1c04996"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.abq0603"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41565\u2010023\u201001404\u20104"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s12276\u2010023\u201001086\u2010x"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41578\u2010021\u201000358\u20100"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2019.01.013"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2307800120"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines9091033"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2020.03.001"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917\u2010023\u201003853\u20104"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2012.04.005"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201701381"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13346\u2010016\u20100282\u20102"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202203022"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41541\u2010025\u201001253\u20103"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2025.125272"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41541\u2010023\u201000732\u20109"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010018\u201030290\u20103"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2102595118"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2182"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(17)30575\u20105"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.09.062"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xphs.2022.11.001"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1080\/17425247.2023.2209718"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2022.06.015"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010023\u201001774\u2010z"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejpb.2025.114811"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13346\u2010025\u201001964\u2010z"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1080\/21645515.2019.1565269"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1201.051237"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.959379"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202202473"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1039\/D3BM01635H"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa043540"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201700326"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2003.12.010"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2021.120586"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2012.69"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1214449110"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.7774\/cevr.2014.3.1.42"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2014.415"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1517\/17425247.2012.676038"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590\u2010022\u201001163\u20109"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.144627"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010022\u201000950\u2010y"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573\u2010021\u201000283\u20105"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1038\/281117a0"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.5.2118"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200737578"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers11030418"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2009.12.005"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010021\u201003653\u20106"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2025.105599"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11307\u2010010\u20100440\u20108"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440\u20101711.2000.00889.x"},{"key":"e_1_2_8_68_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440\u20101711.1998.00709.x"},{"key":"e_1_2_8_69_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep32526"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202522846","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/advs.202522846","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202522846","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T20:53:40Z","timestamp":1783112020000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202522846"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,9]]},"references-count":68,"journal-issue":{"issue":"34","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1002\/advs.202522846"],"URL":"https:\/\/doi.org\/10.1002\/advs.202522846","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,9]]},"assertion":[{"value":"2025-11-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e22846"}}