{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T17:36:29Z","timestamp":1786988189105,"version":"build-2736575974"},"reference-count":69,"publisher":"Wiley","issue":"46","license":[{"start":{"date-parts":[[2026,5,23]],"date-time":"2026-05-23T00:00:00Z","timestamp":1779494400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,23]],"date-time":"2026-05-23T00:00:00Z","timestamp":1779494400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100007428","name":"Naito Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008732","name":"Uehara Memorial Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008732","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Streptavidin, the well\u2010known biotin\u2010binding protein extensively used in biotechnology, is naturally co\u2010produced in\n                    <jats:italic>Streptomyces avidinii<\/jats:italic>\n                    alongside stravidins\u2014inhibitors of biotin biosynthesis. Here, we uncover and activate a conserved genomic region flanked by two streptavidin genes, revealing multiple biosynthetic gene clusters that produce diverse biotin\u2010related metabolites, including stravidins, acidomycin, \u03b1\u2010methyl\u2010KAPA, \u03b1\u2010methyldesthiobiotin, and the novel non\u2010proteinogenic amino acid 2\u2010aminonona\u20105,7\u2010dienedioic acid (ANDA). We show \u03b1\u2010methyldesthiobiotin arises from \u03b1\u2010methyl\u2010KAPA, illustrating how methylated analogues interfere with distinct steps of biotin biosynthesis. Contrary to the view that streptavidin functions solely by sequestering biotin, our biochemical, structural, and bioactivity analyses demonstrate that it also binds acidomycin, neutralizing its antibacterial activity to protect the producer while likely facilitating compound secretion. The crystal structure of the streptavidin\u2013acidomycin complex reveals the molecular basis for this dual functionality. Our findings establish a multifunctional streptavidin that integrates biotin sequestration and self\u2010resistance to balance an antibiotic network targeting the biotin pathway in microbial competition.\n                  <\/jats:p>","DOI":"10.1002\/advs.202523813","type":"journal-article","created":{"date-parts":[[2026,5,23]],"date-time":"2026-05-23T09:09:56Z","timestamp":1779527396000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Streptavidins Coordinate Biotin Sequestration and Self\u2010Resistance Within a Biotin\u2010Pathway Antibiotic Network"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6122-1737","authenticated-orcid":false,"given":"Sumire","family":"Kurosawa","sequence":"first","affiliation":[{"name":"Helmholtz Institute for Pharmaceutical Research Saarland (HIPS) Helmholtz Centre for Infection Research (HZI) Saarbr\u00fccken Germany"},{"name":"Helmholtz International Lab for Anti\u2010Infectives Helmholtz Center for Infection Research Braunschweig Germany"},{"name":"Department of Pharmacy Saarland University Saarbr\u00fccken Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-8222-3354","authenticated-orcid":false,"given":"Jingjun","family":"Mo","sequence":"additional","affiliation":[{"name":"Helmholtz Institute for Pharmaceutical Research Saarland (HIPS) Helmholtz Centre for Infection Research (HZI) Saarbr\u00fccken Germany"},{"name":"Helmholtz International Lab for Anti\u2010Infectives Helmholtz Center for Infection Research Braunschweig Germany"},{"name":"Department of Pharmacy Saarland University Saarbr\u00fccken Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaotong","family":"Diao","sequence":"additional","affiliation":[{"name":"Helmholtz International Lab for Anti\u2010Infectives State Key Laboratory of Microbial Technology Shandong University Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Xie","sequence":"additional","affiliation":[{"name":"Helmholtz Institute for Pharmaceutical Research Saarland (HIPS) Helmholtz Centre for Infection Research (HZI) Saarbr\u00fccken Germany"},{"name":"Helmholtz International Lab for Anti\u2010Infectives Helmholtz Center for Infection Research Braunschweig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Wang","sequence":"additional","affiliation":[{"name":"Helmholtz Institute for Pharmaceutical Research Saarland (HIPS) Helmholtz Centre for Infection Research (HZI) Saarbr\u00fccken Germany"},{"name":"Helmholtz International Lab for Anti\u2010Infectives Helmholtz Center for Infection Research Braunschweig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haowen","family":"Zhao","sequence":"additional","affiliation":[{"name":"Helmholtz Institute for Pharmaceutical Research Saarland (HIPS) Helmholtz Centre for Infection Research (HZI) Saarbr\u00fccken Germany"},{"name":"Helmholtz International Lab for Anti\u2010Infectives Helmholtz Center for Infection Research Braunschweig Germany"},{"name":"Department of Pharmacy Saarland University Saarbr\u00fccken Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dalei","family":"Wu","sequence":"additional","affiliation":[{"name":"Helmholtz International Lab for Anti\u2010Infectives State Key Laboratory of Microbial Technology Shandong University Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rolf","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Helmholtz Institute for Pharmaceutical Research Saarland (HIPS) Helmholtz Centre for Infection Research (HZI) Saarbr\u00fccken Germany"},{"name":"Helmholtz International Lab for Anti\u2010Infectives Helmholtz Center for Infection Research Braunschweig Germany"},{"name":"Department of Pharmacy Saarland University Saarbr\u00fccken Germany"},{"name":"German Centre for Infection Research (DZIF) Braunschweig Germany"},{"name":"PharmaScienceHub Saarbr\u00fccken Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3513-4768","authenticated-orcid":false,"given":"Chengzhang","family":"Fu","sequence":"additional","affiliation":[{"name":"Helmholtz Institute for Pharmaceutical Research Saarland (HIPS) Helmholtz Centre for Infection Research (HZI) Saarbr\u00fccken Germany"},{"name":"Helmholtz International Lab for Anti\u2010Infectives Helmholtz Center for Infection Research Braunschweig Germany"},{"name":"PharmaScienceHub Saarbr\u00fccken Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,23]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-378630-2.00005-0"},{"key":"e_1_2_9_3_1","volume-title":"Posttranslational Modification of 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