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We introduce Polychip, a fully integrated, high\u2010throughput droplet microfluidics platform that revolutionizes microbial interaction screening. By seamlessly combining six microfluidic operations onto a single chip, Polychip achieves 99.7% efficiency and accelerates screening by 11\u201314 times compared to traditional high\u2010throughput liquid handling robotic methods, with minimal human input. Using the Polychip, we screened 2.24\u00a0\u00d7\u00a010\n                    <jats:sup>6<\/jats:sup>\n                    soil\u2010extracted microorganisms against the multidrug\u2010resistant (MDR) pathogen\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    . This process recovered 1.96\u00a0\u00d7\u00a010\n                    <jats:sup>4<\/jats:sup>\n                    hit droplets with an antimicrobial activity confirmation accuracy of 48%. Three environmental isolates (\n                    <jats:italic>Stenotrophomonas<\/jats:italic>\n                    sp.) exhibiting high potency and broad\u2010spectrum antimicrobial activity were identified through this screen. Supernatants from these three environmental isolates suppressed growth of both gram\u2010negative MDR pathogen\n                    <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                    and gram\u2010positive methicillin\u2010resistant\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    (MRSA). Through whole\u2010genome sequencing, metabolic pathway analysis, and mass spectrometry, we identified the secreted compound as enterochelin. This demonstrates Polychip's unprecedented single\u2010cell resolution and high throughput screening capability in rapidly discovering antimicrobial activities, opening new frontiers in combating resistant microbial pathogens, as well as more broadly advancing microbial ecology research.\n                  <\/jats:p>","DOI":"10.1002\/advs.202523854","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T12:08:37Z","timestamp":1785154117000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Polychip\u2010A High\u2010Throughput Droplet Microfluidics Platform for Interrogating Microbial Interactions"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-3972-3166","authenticated-orcid":false,"given":"Jeong Jae","family":"Han","sequence":"first","affiliation":[{"name":"Department of Multidisciplinary Engineering Texas A&amp;M University  College Station Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian Ryan","family":"Guzman","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering Texas A&amp;M University  College Station USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aifen","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Texas A&amp;M University  College Station USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering Texas A&amp;M University  College Station USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rohit","family":"Gupte","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Texas A&amp;M University  College Station USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5441-1663","authenticated-orcid":false,"given":"Haemin","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering Texas A&amp;M University  College Station USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaylee","family":"Delgado","sequence":"additional","affiliation":[{"name":"Department of Microbial Pathogenesis and Immunology Texas A&amp;M University  College Station USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sini","family":"Skariah","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Weill Cornell Medicine \u2013 Qatar Cornell University  Education City Doha Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Sultan","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Weill Cornell Medicine \u2013 Qatar Cornell University  Education City Doha Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arul","family":"Jayaraman","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Texas A&amp;M University  College Station USA"},{"name":"Department of Biomedical Engineering Texas A&amp;M University  College Station USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"de Figueiredo","sequence":"additional","affiliation":[{"name":"Christopher S. 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