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To treat biofilms more effectively, here we discovered a biofilm\u2010binding peptide from a phage library and verified that it selectively bound the polysaccharides on the biofilm. We then engineered M13 phage into trifunctional nanofibers displaying the biofilm\u2010binding peptide at the tip and carrying gold nanoparticles (AuNPs, as photothermal agents) and tetrakis(4\u2010carboxyphenyl) porphyrin (TCPP, as a photosensitizer) on the sidewall for combined phototherapy. This design enhances binding\/anchoring and eradication of biofilms by leveraging the unique properties of each component. The phage nanofibers efficiently bound biofilms and promoted the transfer of heat and penetration of reactive oxygen species (ROS) into the biofilm, leading to cell death. Therefore, under light irradiation, the engineered phage nanofibers effectively eradicated the biofilms by AuNP\u2010induced photothermal therapy (PTT) and TCPP\u2010assisted photodynamic therapy (PDT) in a biofilm\u2010associated skin wound model. Transcriptomic profiling suggests stress\u2010response signatures consistent with oxidative\/thermal injury. This study presents a promising ternary synergistic strategy for eradicating biofilms, potentially improving clinical outcomes in wound infection management.<\/jats:p>","DOI":"10.1002\/advs.202523904","type":"journal-article","created":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T17:24:34Z","timestamp":1783531474000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Biofilm\u2010Binding Phages Enhance Biofilm Eradication by Synergistic Photothermal and Photodynamic Therapy"],"prefix":"10.1002","author":[{"given":"Ying","family":"Cao","sequence":"first","affiliation":[{"name":"Research Center for Analytical Sciences Department of Chemistry College of Sciences Northeastern University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering The Chinese University of Hong Kong  Hong Kong 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