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The early postnatal period constitutes a primary window for muscle fiber transformation. In this study, distinct muscle fiber composition is analyzed by histological and molecular characterization of longissimus dorsi muscle of 2\u2010week\u2010old lean\u2010type and Chinese indigenous pigs. Multi\u2010omics analyses reveal divergence in chromatin states, enhancer landscapes, and promoter activity between breeds. Lean\u2010type pigs show increased chromatin accessibility and enhancer activation at genes involved in oxidative metabolism and myogenesis, whereas Chinese indigenous pigs exhibit enriched regulatory features at glycolytic and biosynthetic loci. Breed\u2010specific SNPs and indels are enriched in a subset of enhancers and potentially influence transcriptional regulation. Higher\u2010order genome architecture further contributes to transcriptional divergence through A\/B compartment switching and topologically associating domain boundary remodeling. Promoter\u2013enhancer interaction mapping reveals breed\u2010specific cis\u2010regulatory networks, and transcriptional output correlates with enhancer density. Functional validation identifies a super\u2010enhancer upstream of\n                    <jats:italic>PPP3CB<\/jats:italic>\n                    that recruits MEF2C to activate oxidative fiber programs. In vitro and in vivo perturbation assays confirm that the\n                    <jats:italic>PPP3CB\u2013<\/jats:italic>\n                    MEF2C feedback loop governs muscle fiber\u2010type specification. Collectively, these findings delineate the epigenomic and 3D genomic architecture underlying early muscle fiber characteristics and provide mechanistic insights relevant to improving meat quality.\n                  <\/jats:p>","DOI":"10.1002\/advs.202523959","type":"journal-article","created":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T12:00:17Z","timestamp":1776427217000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Multi\u2010Omics Insights Into the Mechanisms of Early Muscle Fiber Difference and Transformation Between Lean\u2010Type and Chinese Indigenous Pigs"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7025-0548","authenticated-orcid":false,"given":"Shuailong","family":"Zheng","sequence":"first","affiliation":[{"name":"Key Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture and Rural Affairs Huazhong Agricultural University  Wuhan 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