{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T13:19:31Z","timestamp":1783343971030,"version":"3.54.6"},"reference-count":30,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T00:00:00Z","timestamp":1783296000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T00:00:00Z","timestamp":1783296000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82422040"],"award-info":[{"award-number":["82422040"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Understanding mitochondrial morphology and function is critical for investigating mitochondrial diseases, yet fluorescent probes that label the inner mitochondrial membrane (IMM) and report dysfunction at high spatiotemporal resolution remain limited. Here, we rationally designed aggregation\u2010induced emission\u2010active probes with tunable alkyl chain lengths and carboxyl groups, allowing us to fine\u2011tune phototoxicity and achieve a controlled, mild level of reactive oxygen species (ROS) generation. Two probes, named OTS\u20107C and OTS\u201012C, enabled super\u2011resolution imaging of mitochondrial cristae using stimulated emission depletion microscopy (STED),\u00a0while OTS\u201012C further supported Hessian\u2010structured illumination microscopy (Hessian\u2010SIM) imaging in living cells. Importantly, the controllable ROS output of OTS\u201112C supports prolonged time\u2010lapse imaging of mitochondrial stress responses, including swelling, cristae remodeling, and recovery. We further demonstrated that it could serve as a platform for evaluating antioxidant effectiveness, using Vitamin C and Astaxanthin as model antioxidants. Meanwhile, fluorescence lifetime imaging of OTS\u201012C revealed that ROS\u2010induced oxidation of unsaturated lipids increased its fluorescence lifetime within the IMM, permitting real\u2010time monitoring of mitochondrial functional states. This work presents a simple yet effective strategy to fine\u2010tune the phototoxicity of AIE photosensitizers and provides OTS\u201012C as a versatile fluorescent probe for high\u2010resolution visualization of mitochondrial ultrastructure, investigating mitochondrial stress management and evaluating drug antioxidant efficacy in living cells.<\/jats:p>","DOI":"10.1002\/advs.202524281","type":"journal-article","created":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T12:48:51Z","timestamp":1783342131000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Molecular Engineering Strategy to Fine\u2010Tune Phototoxicity of AIE Probes for Super\u2010Resolution Imaging of Mitochondrial Cristae Dynamics"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5613-6773","authenticated-orcid":false,"given":"Kongqi","family":"Chen","sequence":"first","affiliation":[{"name":"School of Life Sciences The Chinese University of Hong Kong  Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengjie","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Life Sciences The Chinese University of Hong Kong  Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuen","family":"Kam","sequence":"additional","affiliation":[{"name":"School of Life Sciences The Chinese University of Hong Kong  Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiming","family":"Wang","sequence":"additional","affiliation":[{"name":"AIE Institute Key Laboratory of Luminescence from Molecular Aggregates of Guangdong Province State Key Laboratory of Luminescent Materials and Devices South China University of Technology  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinrong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering City University of Hong Kong  Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5263-9189","authenticated-orcid":false,"given":"Sijie","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Life Sciences The Chinese University of Hong Kong  Hong Kong China"},{"name":"AoE Centre for Plant Vacuole Biology and Biotechnology The Chinese University of Hong Kong  Shatin Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,6]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1534\u20105807(01)00055\u20107"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2220"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1241359"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1107402108"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580\u2010020\u20100210\u20107"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2016.01.001"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/bf01059387"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010019\u20101372\u20103"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010biophys\u2010121219\u2010081550"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.4115"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1905924116"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1910574116"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201801350"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202208052"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1039\/d0sc02837a"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2215799119"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.3c11823"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1039\/c7tb02609a"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1039\/c6nr03656b"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.1c01577"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/php.12716"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpcc.5c00119"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41377\u2010023\u201001204\u20104"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.7b00218"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010021\u201003510\u20106"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010017\u201018582\u20106"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010024\u201008146\u2010w"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2012.11.006"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202404328"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.26508\/lsa.201900620"}],"container-title":["Advanced 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