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A multilayer Al\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>3<\/jats:sub>\n                    \/Au\/Si\n                    <jats:sub>3<\/jats:sub>\n                    N\n                    <jats:sub>4<\/jats:sub>\n                    nanopore architecture, produced via helium ion beam lithography, is introduced to address these limitations through structural and materials\u2010level innovation. Finite\u2010element analysis identifies a critical inter\u2010pore spacing approximately 20 times the pore radius as necessary to minimize electric field coupling, enabling rational array design. The membrane structure incorporates a dielectric Al\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>3<\/jats:sub>\n                    layer for electrical isolation and an intermediate gold layer for site\u2010specific aptamer immobilization, confining molecular recognition to the nanopore interior. Arrays with \u223c30\u00a0nm pores and &lt;5% size variation achieve 300\u00a0nm spacing and support statistically independent, parallel signal acquisition. Diverse nanopore arrays with 75\u00a0nm pores and 800\u00a0nm spacing are utilized for the specific detection of alpha\u2010fetoprotein. Detection of alpha\u2010fetoprotein demonstrates label\u2010free sensing at concentrations down to \u223c3\u00a0fM across six orders of magnitude in dynamic range. This platform defines a closed\u2010loop pathway from theoretical modeling to scalable fabrication, establishing a foundation for rational design and high\u2010throughput deployment of solid\u2010state nanopore biosensors.\n                  <\/jats:p>","DOI":"10.1002\/advs.74213","type":"journal-article","created":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T19:23:51Z","timestamp":1770233031000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Tri\u2010Layer Solid\u2010State Nanopore Arrays with Crosstalk Suppression for High\u2010Throughput, Femtomolar\u2010Level Biosensing"],"prefix":"10.1002","volume":"13","author":[{"given":"Silu","family":"Feng","sequence":"first","affiliation":[{"name":"State Key Laboratory of High\u2010Performance Tools Guangdong University of Technology  Guangzhou China"},{"name":"School of Integrated Circuits Guangdong University of Technology  Guangzhou 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