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Structured illumination microscopy (SIM) is widely used for fast super\u2010resolution imaging, yet its sensitivity to scattering strongly restricts volumetric applications. Here we present sparse scanning SIM (SS\u2010SIM), a variant for deep imaging that integrates rapid laser beam scanning with pixel\u2010addressed intensity modulation and sCMOS detection. SS\u2010SIM operates with either one\u2010 or two\u2010photon excitation and achieves a spatial resolution of \u223c150\u00a0nm, and maintains a 1.6\/1.7\u2010fold (lateral\/axial) resolution enhancement over wide\u2010field microscopy throughout the available imaging depth limited by the working distances of our water\u2010immersion objectives (300\/600\u00a0\u00b5m), enabling volumetric visualization of dense biological samples. By combining simplicity, robustness, and super\u2010resolution performance, SS\u2010SIM expands the applicability of SIM to thick tissues, organoids, and small organisms, providing a practical route to high\u2010contrast volumetric imaging in complex biological environments.<\/jats:p>","DOI":"10.1002\/advs.74516","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T17:51:39Z","timestamp":1772992299000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Deep Volumetric Super\u2010Resolution Imaging in Thick Biological Specimens With Sparse Scanning SIM"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4983-7886","authenticated-orcid":false,"given":"Sha","family":"An","sequence":"first","affiliation":[{"name":"School of Physics Xidian University  Xi'an China"},{"name":"Key Laboratory of Optoelectronic Perception of Complex Environment Ministry of 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