{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:25:18Z","timestamp":1786976718410,"version":"build-2736575974"},"reference-count":33,"publisher":"Wiley","issue":"26","license":[{"start":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T00:00:00Z","timestamp":1771977600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T00:00:00Z","timestamp":1771977600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Sclerostin (SOST) is a key negative regulator of bone formation and an emerging biomarker associated not only with osteoporosis but also with chronic kidney disease. Despite its growing clinical relevance, biosensing platforms for SOST remain limited, with most existing approaches relying on antibody\u2010 or aptamer\u2010based assays that often suffer from high cost, limited stability, and reduced performance in complex biological samples. Here, we report a peptide\u2010based electrochemical biosensing platform enabled by a multifunctional zwitterionic biopolymeric nanohydrogel, composed of chitosan, carboxybetaine methacrylate, and gold nanoparticles (CS\u2013ZI\u2013AuNPs). This nanohydrogel acts as a unified antifouling biointerface, simultaneously providing resistance to nonspecific adsorption, stable peptide receptor immobilization, and efficient electrochemical signal transduction. The resulting biosensor achieves picomolar sensitivity with a detection limit of approximately 26.4 pg\/mL, enabling accurate quantification of SOST across clinically relevant concentration ranges. Importantly, the platform demonstrates statistically significant discrimination between healthy individuals and patients with osteoporosis, postmenopausal osteoporosis, and chronic kidney disease (stages 1 and 3) using real clinical samples. By integrating zwitterionic antifouling chemistry with peptide\u2010driven molecular recognition in a conductive nanostructured matrix, this work establishes a generalizable design principle for electrochemical biosensors targeting protein biomarkers. The proposed strategy offers a robust and scalable alternative to conventional immunoassays, with broad implications for translational diagnostics and real\u2010time disease monitoring.<\/jats:p>","DOI":"10.1002\/advs.74575","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T14:50:39Z","timestamp":1772981439000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Peptide Receptor\u2010Functionalized AuNP\u2010Embedded Zwitterionic Biopolymeric Nanohydrogel for Electrochemical Sclerostin Sensing"],"prefix":"10.1002","volume":"13","author":[{"given":"Hyo Jeong","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Food Science and Technology and GreenTech\u2010Based Food Safety Research Group BK21 Four Chung\u2010Ang University  Anseong Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae Hwan","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology and GreenTech\u2010Based Food Safety Research Group BK21 Four Chung\u2010Ang University  Anseong Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingi","family":"Jo","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Chung\u2010Ang University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changyeon","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering Chung\u2010Ang University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7750-9679","authenticated-orcid":false,"given":"Hyunmin","family":"Yi","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering Tufts University  Medford Massachusetts United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-2631-6038","authenticated-orcid":false,"given":"Al\u2010Montaser Bellah H.","family":"Ali","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry Faculty of Pharmacy Assiut University  Assiut Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4119-1574","authenticated-orcid":false,"given":"Jong Pil","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology and GreenTech\u2010Based Food Safety Research Group BK21 Four Chung\u2010Ang University  Anseong Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm11030806"},{"key":"e_1_2_8_3_1","article-title":"Low Levels of Serum Sclerostin in Adult Patients With Tumor\u2010Induced Osteomalacia","volume":"2022","author":"Chen L.","year":"2022","journal-title":"Disease Markers"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00198-022-06523-7"},{"key":"e_1_2_8_5_1","volume":"14","author":"Neto R.","year":"2021","journal-title":"Clin. 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