{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T21:21:30Z","timestamp":1783113690004,"version":"3.54.6"},"reference-count":55,"publisher":"Wiley","issue":"34","license":[{"start":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T00:00:00Z","timestamp":1775779200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T00:00:00Z","timestamp":1775779200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC3401100"],"award-info":[{"award-number":["2022YFC3401100"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2024YFC2707404"],"award-info":[{"award-number":["2024YFC2707404"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["92354305 32271428 32201132 82372672 31600692"],"award-info":[{"award-number":["92354305 32271428 32201132 82372672 31600692"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Blood\u2010based liquid biopsies hold transformative potential for non\u2010invasive cancer management, but current approaches relying on rare circulating tumor components limit their broad clinical utility. Platelets, abundant in blood and mediating diverse cancer\u2010associated responses, represent a compelling yet largely unexplored alternative. Using SIM super\u2010resolution microscopy, we analyzed \u03b1\u2010granule distributions in platelets from a multicenter cohort (\n                    <jats:italic>n<\/jats:italic>\n                    = 1,556) encompassing nine cancer types and twelve non\u2010malignant diseases. We identified robust cancer\u2010associated alterations, particularly a marked increase in the \u201cCircle\u201d pattern, which exhibits excellent multi\u2010cancer diagnostic potential. This method, termed PAID (Platelet Alpha\u2010granule Imaging\u2010based Diagnostic assay), achieved 85.0% accuracy in prostate cancer within the diagnostically challenging PSA \u201cgray zone\u201d. PAID combined with PSA improved diagnostic accuracy to 94.2%, 38.4% over PSA alone, suggesting significant synergy. In ovarian cancer, PAID combined with HE4 enhanced diagnostic accuracy from 73.8% to 88.3%. Furthermore, PAID outperformed CA125 in sensitivity (90.3%\u00a0vs 60.0%) for detecting ovarian cancer recurrence. We also observed that tumor cells may utilize both tumor\u2010derived exosomes and proteins to remodel platelet \u03b1\u2010granule distributions, suggesting the increased \u201cCircle\u201d pattern arises from synergistic rather than singular triggers. The non\u2010invasive, highly accurate PAID shows great promise in advancing liquid biopsy for cancer management.\n                  <\/jats:p>","DOI":"10.1002\/advs.75094","type":"journal-article","created":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T14:58:13Z","timestamp":1775833093000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Large\u2010Scale Quantitative Morphometry of Platelet \u03b1\u2010Granules via SIM Super\u2010Resolution Microscopy for Cancer Liquid Biopsy"],"prefix":"10.1002","volume":"13","author":[{"given":"Yan","family":"Ma","sequence":"first","affiliation":[{"name":"MOE Key Laboratory for Biomedical Photonics Advanced Biomedical Imaging Facility\u2010Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology  Wuhan China"},{"name":"Institute of Blood Transfusion of Hubei Province Wuhan Blood Center  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2446-5254","authenticated-orcid":false,"given":"Huan","family":"Deng","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory for Biomedical Photonics Advanced Biomedical Imaging Facility\u2010Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology  Wuhan China"},{"name":"Institute of Blood Transfusion of Hubei Province Wuhan Blood Center  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8098-4235","authenticated-orcid":false,"given":"Zelin","family":"Liu","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory for Biomedical Photonics Advanced Biomedical Imaging Facility\u2010Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simei","family":"Zhong","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory for Biomedical Photonics Advanced Biomedical Imaging Facility\u2010Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liangliang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6745-5442","authenticated-orcid":false,"given":"Mu","family":"He","sequence":"additional","affiliation":[{"name":"Department of Urology Renmin Hospital of Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Jing","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory for Biomedical Photonics Advanced Biomedical Imaging Facility\u2010Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihua","family":"Dai","sequence":"additional","affiliation":[{"name":"Institute of Blood Transfusion of Hubei Province Wuhan Blood Center  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuzhen","family":"Basang","sequence":"additional","affiliation":[{"name":"Hepatic Surgery Center Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010Yu","family":"Hu","sequence":"additional","affiliation":[{"name":"Institute of Blood Transfusion of Hubei Province Wuhan Blood Center  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changyi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huimin","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoren","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Urology Renmin Hospital of Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe","family":"Yang","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory for Biomedical Photonics Advanced Biomedical Imaging Facility\u2010Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanshan","family":"Zhao","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory for Biomedical Photonics Advanced Biomedical Imaging Facility\u2010Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohai","family":"Hu","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory for Biomedical Photonics Advanced Biomedical Imaging Facility\u2010Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanjie","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowei","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Xu","sequence":"additional","affiliation":[{"name":"Institute of Blood Transfusion of Hubei Province Wuhan Blood Center  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoqun","family":"Zeng","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory for Biomedical Photonics Advanced Biomedical Imaging Facility\u2010Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9050-4496","authenticated-orcid":false,"given":"Jing","family":"Yuan","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory for Biomedical Photonics Advanced Biomedical Imaging Facility\u2010Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Li","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory for Biomedical Photonics Advanced Biomedical Imaging Facility\u2010Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziwei","family":"Qu","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery Traditional Chinese and Western Medicine Hospital of Wuhan Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4064-8907","authenticated-orcid":false,"given":"Zhenya","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Hematology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingjuan","family":"Chen","sequence":"additional","affiliation":[{"name":"Cancer Center Union Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Hubei Key Laboratory of Precision Radiation Oncology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8036-0412","authenticated-orcid":false,"given":"Hanhua","family":"Dong","sequence":"additional","affiliation":[{"name":"Hepatic Surgery Center Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Department of Hepatobiliary Surgery Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences Tongji Shanxi Hospital Third Hospital of Shanxi Medical University  Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Urology Renmin Hospital of Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiqiang","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology  Wuhan China"},{"name":"Department of Obstetrics and Gynecology Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences Tongji Shanxi Hospital Third Hospital of Shanxi Medical University  Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5158-0645","authenticated-orcid":false,"given":"Yu\u2010Hui","family":"Zhang","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory for Biomedical Photonics Advanced Biomedical Imaging Facility\u2010Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-021-00837-3"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0703-0"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571-020-00457-x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xcrm.2023.101198"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.01.030"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aar3247"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aan2415"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.adf2341"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-022-01505-z"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2022.983724"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41568-023-00639-6"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2019003978"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2019003976"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41569-018-0110-0"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/smtd.202300445"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1518628112"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23116022"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1142\/S1793545824300064"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12951-024-02605-2"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtha.2023.01.006"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-89799-9"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2018.3712"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jlb.2025.100305"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers14133272"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198710083171501"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-022-01527-7"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.4103\/1008-682X.150846"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2016.08.103"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajur.2015.04.022"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/pros.24752"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2014.08.010"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-022-00860-y"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(15)01224-6"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(21)00731-5"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2013.11.030"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms231912041"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(18)30837-4"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2589-7500(21)00278-8"},{"key":"e_1_2_9_40_1","first-page":"579","article-title":"Platelet RNA Enables Accurate Detection of Ovarian Cancer: An Intercontinental, Biomarker Identification Study","volume":"14","author":"Gao Y.","year":"2023","journal-title":"Protein Cell"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.21037\/gs-20-544"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2022.09.004"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2022.08.006"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/1878-0261.13014"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.adp3971"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21020602"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.5440"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.122.321861"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-023-01662-3"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40364-018-0118-y"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2015.09.018"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-024-03026-7"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-11-679852"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.1173"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcp1000274"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-46700-2"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.75094","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/advs.75094","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.75094","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T20:53:50Z","timestamp":1783112030000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.75094"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,10]]},"references-count":55,"journal-issue":{"issue":"34","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1002\/advs.75094"],"URL":"https:\/\/doi.org\/10.1002\/advs.75094","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,10]]},"assertion":[{"value":"2026-02-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e75094"}}