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Here, we propose femtosecond\u2010laser\u2010induced periodic phase\u2010transition (\n                    <jats:italic>fs<\/jats:italic>\n                    \u2010LIPPT) of 1T\u2019\u2010MoTe\n                    <jats:sub>2<\/jats:sub>\n                    nanograting template, inducing Au nanostructures for frequency\u2010shift digital\u2010SERS immunoassay. The interference between the incident transverse electric wave and propagation wave in the 2H\u2010MoTe\n                    <jats:sub>2<\/jats:sub>\n                    \/SiO\n                    <jats:sub>2<\/jats:sub>\n                    \u2010interlayer waveguide triggers the highly homogeneous periodic 1T\u2019 phase\u2010transition pattern. The Au nanoparticles (Au\n                    <jats:italic>NP<\/jats:italic>\n                    s) are subsequently reduced at the 1T\u2019 regions in chloroauric acid solution, forming a subwavelength Au\n                    <jats:italic>NP<\/jats:italic>\n                    s@1T\u2019\u2010MoTe\n                    <jats:sub>2<\/jats:sub>\n                    nanograting with 295.0 \u00b1 8.1\u00a0nm in period. The Fano resonances by coupling the narrow\u2010band guided\u2010mode resonances supported in the 1T\u2019\u2010MoTe\n                    <jats:sub>2<\/jats:sub>\n                    \/SiO\n                    <jats:sub>2<\/jats:sub>\n                    \u2010interlayer nanograting with the broadband LSPRs in Au\n                    <jats:italic>NP<\/jats:italic>\n                    s promote optical localization, achieving the superior performance with a limit of detection in 10\n                    <jats:sup>\u221214<\/jats:sup>\n                    \u00a0\n                    <jats:sc>m<\/jats:sc>\n                    and a SERS performance factor of 7.5 \u00d7 10\n                    <jats:sup>6<\/jats:sup>\n                    for rhodamine 6G (R6G). A paradigm of frequency\u2010shift digital\u2010SERS immunoassay for serological diagnosis is established using the R6G\u2010labeled Au\n                    <jats:italic>NP<\/jats:italic>\n                    s@1T\u2019\u2010MoTe\n                    <jats:sub>2<\/jats:sub>\n                    nanograting conjugated with immunoglobulin G of rheumatoid arthritis, by which the discrimination accuracy is unexpectedly close to unity. The present work provides a novel protocol for SERS serum immunoassay, opening opportunities for early diagnosis and accurate prognosis of autoimmune diseases by blood test in the future.\n                  <\/jats:p>","DOI":"10.1002\/advs.75366","type":"journal-article","created":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T19:16:31Z","timestamp":1776366991000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Laser\u2010Induced Periodic Phase\u2010Transition of 2D\u2010MoTe\n                    <sub>2<\/sub>\n                    Nanograting Template for Frequency\u2010Shift Digital\u2010SERS Immunoassay of Autoimmune Disease"],"prefix":"10.1002","volume":"13","author":[{"given":"Yao","family":"Yao","sequence":"first","affiliation":[{"name":"School of Physics and Optoelectronic Engineering Beijing University of Technology  Beijing 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