{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T17:04:08Z","timestamp":1786122248220,"version":"build-2736575974"},"reference-count":33,"publisher":"Wiley","issue":"44","license":[{"start":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T00:00:00Z","timestamp":1778630400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T00:00:00Z","timestamp":1778630400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22374045"],"award-info":[{"award-number":["22374045"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Precise tumor imaging is essential for accurate intraoperative decision\u2010making, thereby directly influencing patient prognosis. Optical molecular probes enabling non\u2010invasive, dynamic assessment of cancerous lesions are clinically crucial. However, current optical molecular probes face challenges in dodging false\u2010positive and false\u2010negative signals at once during imaging, limiting their clinical diagnostic use. Here, we introduce a class of lysosome\u2010targeted, activatable optical probes (Dx\u2010NH\n                    <jats:sub>2<\/jats:sub>\n                    ), leveraging the increased lysosomal abundance during tumor metabolic reprogramming. Lysosomal protonation retains probes for better single\u2010cell resolution and fewer false\u2010negative signals. To enable more high\u2010precision tumor imaging, we synthesized probe CN\u2010D\u2010GGT from CN\u2010D\u2010NH\n                    <jats:sub>2<\/jats:sub>\n                    by introducing a \u03b3\u2010glutamyltransferase substrate, following our group's prior offensive and defensive integration strategy. Due to its smart design, CN\u2010D\u2010GGT, upon activation, shows marked changes in fluorescence and photoacoustic signals, enabling its application for multi\u2010modal imaging. It also has high specificity, distinguishing cancer cells in co\u2010culture (\u223c6\u2010fold) and clearly differentiating tumors from normal and inflamed tissues (T\/N or T\/I signal ratios &gt; 3.5). Importantly, it can also differentiate cancerous from adjacent tissues in clinical samples. This work has developed a probe that can accurately light tumors in complex pathological environments, with the potential to assist in intraoperative resection decision\u2010making, avoid excessive or missed resection.\n                  <\/jats:p>","DOI":"10.1002\/advs.75664","type":"journal-article","created":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T15:05:21Z","timestamp":1778684721000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Synergistic Probe Combining Lysosome Anchoring and Tumor Defense System Targeting for Precise Tumor Visualization"],"prefix":"10.1002","volume":"13","author":[{"given":"Yang","family":"Shen","sequence":"first","affiliation":[{"name":"Department of Hepatobiliary Surgery Central Laboratory Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University)  Changsha P. R. China"},{"name":"State Key Laboratory of Chemo and Biosensing College of Chemistry and Chemical Engineering Hunan University  Changsha P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiawei","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chemo and Biosensing College of Chemistry and Chemical Engineering Hunan University  Changsha P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruixi","family":"Yi","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chemo and Biosensing College of Chemistry and Chemical Engineering Hunan University  Changsha P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Shi","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chemo and Biosensing College of Chemistry and Chemical Engineering Hunan University  Changsha P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chemo and Biosensing College of Chemistry and Chemical Engineering Hunan University  Changsha P. R. China"},{"name":"School of Chemistry and Molecular Engineering Nanjing Tech University  Nanjing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5257-3922","authenticated-orcid":false,"given":"Sulai","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery Central Laboratory Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University)  Changsha P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1015-5319","authenticated-orcid":false,"given":"Lin","family":"Yuan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chemo and Biosensing College of Chemistry and Chemical Engineering Hunan University  Changsha P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,13]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21871"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1CS00543J"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1CS00525A"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/ange.202209512"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.3c00612"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2025.216549"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41570\u2010021\u201000277\u20102"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/asia.202401647"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9SC03628H"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscentsci.9b00678"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chempr.2020.10.010"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1039\/D5CS00984G"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c04793"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1160809"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202515403"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590\u2010023\u201001719\u20103"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010018\u201005261\u2010x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010020\u201016814\u20104"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12692"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(76)80047-6"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja305802v"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202406332"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2021.130786"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010physiol\u2010021119\u2010034627"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202003757"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201907778"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2005.10.005"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2023.09.020"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5c00392"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2023.134718"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.2c04894"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41568\u2010024\u201000786\u20104"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01322"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.75664","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/advs.75664","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.75664","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T09:07:17Z","timestamp":1786093637000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.75664"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,13]]},"references-count":33,"journal-issue":{"issue":"44","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/advs.75664"],"URL":"https:\/\/doi.org\/10.1002\/advs.75664","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,13]]},"assertion":[{"value":"2026-01-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e75664"}}