{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T17:36:32Z","timestamp":1786988192194,"version":"build-2736575974"},"reference-count":38,"publisher":"Wiley","issue":"46","license":[{"start":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T00:00:00Z","timestamp":1779148800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T00:00:00Z","timestamp":1779148800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Multi\u2010subunit formate dehydrogenases (FDHs), which catalyze the interconversion of formate and carbon dioxide (CO\n                    <jats:sub>2<\/jats:sub>\n                    ), have drawn increasing attention for mitigating climate change and advancing environmental protection owing to their advantages of oxygen tolerance and easy heterogenous expression. However, differently sourced multi\u2010subunit FDHs exhibit distinct catalytic biases, and the reasons remain unclear. On the basis of the exceptional observation of\n                    <jats:italic>Rhodobacter aestuarii<\/jats:italic>\n                    FDH favoring CO\n                    <jats:sub>2<\/jats:sub>\n                    reduction, this study unveiled an oxidation inhibition effect in exclusively NADH\/NAD\n                    <jats:sup>+<\/jats:sup>\n                    \u2010involved catalysis via kinetics analysis in terms of different redox couples. Substrate truncation positioned Fdh\u03b2 as the predominant subunit. Further studies based on structural and electrochemical insights interpreted that the slow desorption of NADH is the underlying determinant for the apparent catalytic bias. Knowledge\u2010based rational design helped obtain a beneficial variant,\n                    <jats:italic>Ra<\/jats:italic>\n                    FDH \u03b2 E260Y, with a 10\u2010fold increased catalytic activity in CO\n                    <jats:sub>2<\/jats:sub>\n                    reduction, highlighting its potential for CO\n                    <jats:sub>2<\/jats:sub>\n                    biotransformation and applications in low\u2010carbon biomanufacturing. Eventually, bioinformatic analysis suggested that the diaphorase\u2010like subunits and the catalysis regulation mechanism may widely exist in living organisms for modulating the redox balance of oxidoreductases, providing new insights into metabolism and catabolism.\n                  <\/jats:p>","DOI":"10.1002\/advs.75764","type":"journal-article","created":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T15:11:08Z","timestamp":1779203468000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Understanding the Catalytic Determinant role of Diaphorase\u2010Like Subunit in Formate Dehydrogenases via Redox Couples"],"prefix":"10.1002","volume":"13","author":[{"given":"Kuncheng","family":"Zhang","sequence":"first","affiliation":[{"name":"University of Chinese Academy of Sciences  Beijing China"},{"name":"State Key Laboratory of Engineering Biology for Low\u2010carbon Manufacturing Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weisong","family":"Liu","sequence":"additional","affiliation":[{"name":"University of Chinese Academy of Sciences  Beijing China"},{"name":"State Key Laboratory of Engineering Biology for Low\u2010carbon Manufacturing Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Su","sequence":"additional","affiliation":[{"name":"University of Chinese Academy of Sciences  Beijing China"},{"name":"State Key Laboratory of Engineering Biology for Low\u2010carbon Manufacturing Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3489-1097","authenticated-orcid":false,"given":"Huijuan","family":"Cui","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Engineering Biology for Low\u2010carbon Manufacturing Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanming","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Engineering Biology for Low\u2010carbon Manufacturing Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6625-5087","authenticated-orcid":false,"given":"Zhiguang","family":"Zhu","sequence":"additional","affiliation":[{"name":"University of Chinese Academy of Sciences  Beijing China"},{"name":"State Key Laboratory of Engineering Biology for Low\u2010carbon Manufacturing Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun","family":"You","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Metabolism School of Life Sciences and Biotechnology Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3384-2576","authenticated-orcid":false,"given":"Lingling","family":"Zhang","sequence":"additional","affiliation":[{"name":"University of Chinese Academy of Sciences  Beijing China"},{"name":"State Key Laboratory of Engineering Biology for Low\u2010carbon Manufacturing Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,19]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2021.01.003"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5CS00182J"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.2c00581"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10930-021-10007-8"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcou.2020.101353"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2021.07.011"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2019.06.007"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1244758"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13068-018-1236-3"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2020.123155"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-15614-0"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.12528"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.7.1864-1871.2000"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja508647u"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201803397"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0801290105"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1033.2003.03619.x"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.0c00086"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.6b03941"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1039\/D2CC01541B"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2025.133029"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2024.10.007"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-61001-y"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2008.08.026"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-46909-1"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1872-2067(23)64445-6"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-026-69827-w"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aan4497"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2016.08.008"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijhydene.2011.06.078"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.21.6344-6354.2001"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.004507-0"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms14049"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2015.08.008"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.42166"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444910007493"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444909052925"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.3235"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.75764","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/advs.75764","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.75764","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T17:16:42Z","timestamp":1786987002000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.75764"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,19]]},"references-count":38,"journal-issue":{"issue":"46","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/advs.75764"],"URL":"https:\/\/doi.org\/10.1002\/advs.75764","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,19]]},"assertion":[{"value":"2026-01-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e75764"}}