{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:05:40Z","timestamp":1788998740340,"version":"build-2803163510"},"reference-count":49,"publisher":"Wiley","issue":"46","license":[{"start":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T00:00:00Z","timestamp":1779148800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T00:00:00Z","timestamp":1779148800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Pseudorabies virus (PRV), an\u00a0\u03b1\u2011herpesvirus, has recently been recognized as a potentially significant zoonotic pathogen, causing severe encephalitis and endophthalmitis in humans with high mortality and disability rates. Despite its growing public\u2011health threat, no effective therapeutics are available for human PRV infection. Here, we isolated a broadly neutralizing monoclonal antibody, designated 6F7, which targets the PRV glycoprotein\u00a0D (gD) and neutralizes all tested PRV lineages. Mechanistic studies reveal that 6F7 does not impede viral attachment or internalization; instead, it specifically blocks the fusion of the viral envelope with cellular membranes, thereby preventing viral replication. Moreover, the antibody also potently suppresses cell\u2011to\u2011cell spread of PRV. We demonstrate that the interaction between gD and the PRV receptor Nectin\u20111 is blocked by 6F7. Last, a humanized version of 6F7 confers complete protection in mice challenged with a lethal PRV variant and blocks the establishment of latency at a dose of 15\u00a0mg\/kg per mouse. Overall, the humanized broadly neutralizing antibody represents a promising therapeutic candidate for human PRV infection.<\/jats:p>","DOI":"10.1002\/advs.75771","type":"journal-article","created":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T14:51:09Z","timestamp":1779202269000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A Humanized Anti\u2010gD Broadly Neutralizing Antibody Confers Complete Post\u2010Exposure Protection Against Pseudorabies Virus"],"prefix":"10.1002","volume":"13","author":[{"given":"Yue","family":"Sun","sequence":"first","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianbo","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and 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