{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T17:36:23Z","timestamp":1786988183943,"version":"build-2736575974"},"reference-count":30,"publisher":"Wiley","issue":"46","license":[{"start":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T00:00:00Z","timestamp":1779840000000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T00:00:00Z","timestamp":1779840000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T00:00:00Z","timestamp":1779840000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["APP1185907"],"award-info":[{"award-number":["APP1185907"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["APP2039176"],"award-info":[{"award-number":["APP2039176"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["FL220100059"],"award-info":[{"award-number":["FL220100059"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001111","name":"Cancer Australia","doi-asserted-by":"publisher","award":["2010799"],"award-info":[{"award-number":["2010799"]}],"id":[{"id":"10.13039\/501100001111","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Digital surface\u2010enhanced Raman scattering (SERS) immunoassays digitize epitope binding events to achieve ultrasensitive protein detection. However, existing implementations predominantly rely on single\u2010epitope recognition, yielding 1D molecular view of antigen structure and obscuring how conformational heterogeneity or mutation\u2010induced changes affect epitopes. This limitation cannot be resolved simply by combining multiple monoclonal antibodies, as heterogeneous conjugation and uncontrolled binding collapse epitope\u2010specific responses into pooled, non\u2010assignable signals. Here, we introduce EpiCount\u2010SERS (Epitope\u2010Resolved Digital Counting by SERS), a multi\u2010epitope digital SERS framework that enables epitope\u2010resolved immunochemical profiling. The platform employs nanobody\u2010based bispecific antibody fragments that pair epitope\u2010specific nanobodies with a unified anti\u2010methoxy polyethylene glycol conjugation domain, enabling orientation\u2010controlled attachment to spectrally encoded SERS nanotags. Epitope\u2010specific binding events are discretized into independent digital channels, allowing distinct epitopes on the SARS\u2010CoV\u20102 receptor\u2010binding domain to be interrogated in parallel. Digital enumeration across these channels generates epitope\u2010resolved molecular fingerprints that capture information beyond single\u2010epitope assays. EpiCount\u2010SERS achieves sub\u2010ng mL\n                    <jats:sup>\u2212<\/jats:sup>\n                    <jats:sup>1<\/jats:sup>\n                    sensitivity for recombinant protein, detects inactivated virus at approximately 10\n                    <jats:sup>2<\/jats:sup>\n                    copies \u00b5L\n                    <jats:sup>\u2212<\/jats:sup>\n                    <jats:sup>1<\/jats:sup>\n                    , and classifies clinical nasopharyngeal swab samples with accuracy of 88.3% (area under the curve = 0.9467). Because epitope binders can be exchanged without altering the unified conjugation strategy, EpiCount\u2010SERS provides a scalable framework for digital immunochemical profiling of structurally dynamic protein targets.\n                  <\/jats:p>","DOI":"10.1002\/advs.75828","type":"journal-article","created":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T15:12:22Z","timestamp":1779894742000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Epitope\u2010Resolved Digital SERS Profiling of Structurally Dynamic Antigens via a Multi\u2010Epitope Bispecific Antibody Framework"],"prefix":"10.1002","volume":"13","author":[{"given":"Jing","family":"Wang","sequence":"first","affiliation":[{"name":"Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education Fujian Provincial Key Laboratory of Photonics Technology Fujian Normal University  Fuzhou China"},{"name":"Australian Institute for Bioengineering and Nanotechnology (AIBN) The University of Queensland  Brisbane QLD Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Australian Institute for Bioengineering and Nanotechnology (AIBN) The University of Queensland  Brisbane QLD Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kym","family":"Lowry","sequence":"additional","affiliation":[{"name":"Frazer Institute Faculty of Health, Medicine, and Behavioural Sciences The University of Queensland Royal Brisbane and Women's Hospital Brisbane QLD Australia"},{"name":"Queensland Paediatric Infectious Diseases (QPID) Sakzewski Laboratory Queensland Children's Hospital  Brisbane QLD Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher B.","family":"Howard","sequence":"additional","affiliation":[{"name":"Australian Institute for Bioengineering and Nanotechnology (AIBN) The University of Queensland  Brisbane QLD Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matt","family":"Trau","sequence":"additional","affiliation":[{"name":"Australian Institute for Bioengineering and Nanotechnology (AIBN) The University of Queensland  Brisbane QLD Australia"},{"name":"School of Chemistry and Molecular Biosciences The University of Queensland  Brisbane QLD Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,27]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.2c00316"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1039\/D4CS00090K"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.8b10112"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2024.116973"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.2c07497"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsanm.3c01979"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/smsc.202400259"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2025.117216"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2025.117292"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/D4SD00206G"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2021.338907"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1142"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.abm1262"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2014.08.032"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12951\u2010020\u201000680\u20109"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5c03980"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3001236"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abe4747"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201600263"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202010296"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202401818"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssensors.1c00358"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-24963-3"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0030237"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abb7269"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1080\/19420862.2021.1993768"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssensors.1c02664"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.120.15256"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41422\u2010022\u201000700\u20103"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010021\u201024963\u20103"}],"container-title":["Advanced 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