{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T18:27:35Z","timestamp":1787596055734,"version":"build-2736575974"},"reference-count":49,"publisher":"Wiley","issue":"47","license":[{"start":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T00:00:00Z","timestamp":1781568000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T00:00:00Z","timestamp":1781568000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"publisher","award":["202206280025"],"award-info":[{"award-number":["202206280025"]}],"id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Leader and follower cells drive collective cancer cell migration through distinct and interactive roles, but their specific metabolic signatures remain largely unknown. Here, we developed and applied a workflow that integrates time\u2010lapse imaging, single\u2010cell tracking, microsampling and direct infusion high\u2010resolution mass spectrometry to profile the lipidomes of individually sampled leader and follower cells from highly migratory TNBC lines HCC1143 and HCC38. Approximately 120 metabolites were detected per cell at the MS\n                    <jats:sup>1<\/jats:sup>\n                    level, and 70 compounds were assigned based on matched MS\n                    <jats:sup>2<\/jats:sup>\n                    fragments, indicative of detected head\u2010group or side\u2010chain fragment ions. Distinct leader\u2010follower differences emerged in each cell line. HCC38 cells showed increased fatty acids (FA) in leader cells. In addition, alterations in several phosphatidylethanolamine (PE) and diacylglycerol (DG) species between leader and follower cell subpopulations were detected. Alternatively, leader and follower cells in HCC1143 exhibited changes in phosphatidylcholine (PC) species, with PC(34:1) confirmed by a fragment ions at m\/z 184.0733. Overall, this study revealed the dynamic lipid metabolism in live migratory cancer cells, and highlights the potential of integrated dynamic imaging and single\u2010cell mass spectrometry for studying functional heterogeneity within leader\u2010follower cancer phenotypes.\n                  <\/jats:p>","DOI":"10.1002\/advs.75862","type":"journal-article","created":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T15:33:04Z","timestamp":1781623984000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Imaging\u2010Guided Live Single\u2010Cell Lipid Profiling of Leader and Follower Cells During Collective Migration of Triple\u2010Negative Breast Cancer Cells"],"prefix":"10.1002","volume":"13","author":[{"given":"Xiaoyue","family":"Huang","sequence":"first","affiliation":[{"name":"Metabolomics and Analytics Centre Leiden Academic Centre For Drug Research Leiden University  Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judit Gonz\u00e1lez","family":"Riera","sequence":"additional","affiliation":[{"name":"Metabolomics and Analytics Centre Leiden Academic Centre For Drug Research Leiden University  Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mai","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Metabolomics and Analytics Centre Leiden Academic Centre For Drug Research Leiden University  Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy","family":"Broekhuis","sequence":"additional","affiliation":[{"name":"Institute of Biology Leiden University  Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0615-9616","authenticated-orcid":false,"given":"Sylvia E. Le","family":"D\u00e9v\u00e9dec","sequence":"additional","affiliation":[{"name":"Division of Cell Systems and Drug Safety, Leiden Academic Centre For Drug Research Leiden University  Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2157-4399","authenticated-orcid":false,"given":"Ahmed","family":"Ali","sequence":"additional","affiliation":[{"name":"Metabolomics and Analytics Centre Leiden Academic Centre For Drug Research Leiden University  Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Hankemeier","sequence":"additional","affiliation":[{"name":"Metabolomics and Analytics Centre Leiden Academic Centre For Drug Research Leiden University  Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,16]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2015.07.005"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-06-1109"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrclinonc.2016.66"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010024\u201001885\u20102"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2720"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2548"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.11.016"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2015.06.003"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41568\u2010021\u201000376\u20108"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plipres.2025.101330"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.11.029"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep07656"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms15078"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.11.029"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045\u2010023\u201001498\u20102"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010020\u201015219\u20107"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201911120"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41389\u2010022\u201000420\u20108"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.CAN\u201023\u20102491"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.CAN\u201009\u20102305"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102407\u201011\u2010465"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2025.159679"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.11560"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5c02010"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1039\/D5AN00851D"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010021\u201023161\u20105"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592\u2010024\u201002392\u20106"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201048865\u20102"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2015.084"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2024.127127"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.R800095\u2010JLR200"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2023.464342"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.1777"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq054"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/2047\u2010217X\u20102\u201013"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.854721"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.201801158"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gendis.2024.101491"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/gastro\/goaa066"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.768606"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2017.04.006"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/4829180"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0205706"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010025\u201057439\u20109"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13184699"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2023.5563"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/embor.2012.149"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms251910554"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0305491"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.75862","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/advs.75862","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.75862","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T17:44:20Z","timestamp":1787593460000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.75862"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,16]]},"references-count":49,"journal-issue":{"issue":"47","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/advs.75862"],"URL":"https:\/\/doi.org\/10.1002\/advs.75862","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,16]]},"assertion":[{"value":"2026-01-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e75862"}}