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Although numerous transcription factors have been linked to drought responses, the regulatory networks underlying drought tolerance in rice remain incompletely understood. Here, through a CRISPR\/Cas9\u2010based genetic screen of a rice mutant population, we identified a mutant with decreased drought tolerance,\n                    <jats:italic>osdt2<\/jats:italic>\n                    .\n                    <jats:italic>OsDT2<\/jats:italic>\n                    encodes a CCCH\u2010type zinc\u2010finger protein and its overexpression confers strong drought resistance. We demonstrate that OsDT2 physically interacts with the bZIP transcription factor OsbZIP66, and they synergistically activate drought\u2010responsive genes, such as\n                    <jats:italic>LATE EMBRYOGENESIS ABUNDANT 3<\/jats:italic>\n                    (\n                    <jats:italic>OsLEA3<\/jats:italic>\n                    ), to enhance drought tolerance. Furthermore, the nuclear factor Y subunit C4 (OsNF\u2010YC4) interacts with both OsDT2 and OsbZIP66, coordinating their recruitment to the promoter of\n                    <jats:italic>OsLEA3<\/jats:italic>\n                    . Remarkably, the OsDT2 function is conserved across cereals, including wheat and maize, and the tripartite DT2\u2010bZIP66\u2010NF\u2010YC4 module is functionally conserved in mediating drought responses in both rice and\n                    <jats:italic>Arabidopsis<\/jats:italic>\n                    . Collectively, our study reveals a novel, evolutionarily conserved transcriptional regulatory module that fine\u2010tunes drought\u2010responsive gene expression and provides potential targets for engineering drought\u2010resilient crops.\n                  <\/jats:p>","DOI":"10.1002\/advs.76034","type":"journal-article","created":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T10:14:23Z","timestamp":1781000063000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A Conserved DT2\u2010bZIP66\u2010NF\u2010YC4 Regulatory Module Confers Drought Tolerance in Rice and\n                    <i>Arabidopsis<\/i>"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5805-3514","authenticated-orcid":false,"given":"Jun","family":"Shen","sequence":"first","affiliation":[{"name":"State Key Laboratory of Rice Biology and Breeding, Zhejiang Provincial Key Laboratory of Crop Genetic Resources, College of Agriculture and Biotechnology Zhejiang University  Hangzhou China"},{"name":"College of Agronomy, Bio\u2010breeding Laboratory of Anhui Province Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuchen","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology and Breeding, Zhejiang Provincial Key Laboratory of Crop Genetic Resources, College of Agriculture and Biotechnology Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8726-1128","authenticated-orcid":false,"given":"Liang","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Agronomy, Bio\u2010breeding Laboratory of Anhui Province Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaosong","family":"Ma","sequence":"additional","affiliation":[{"name":"Shanghai Agrobiological Gene Center  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenyin","family":"Peng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology and Breeding, Zhejiang Provincial Key Laboratory of Crop Genetic Resources, College of Agriculture and Biotechnology Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunting","family":"Yuan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology and Breeding, Zhejiang Provincial Key Laboratory of Crop Genetic Resources, College of Agriculture and Biotechnology Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Wu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology and Breeding, Zhejiang Provincial Key Laboratory of Crop Genetic Resources, College of Agriculture and Biotechnology Zhejiang 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