{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T17:36:25Z","timestamp":1786988185537,"version":"build-2736575974"},"reference-count":38,"publisher":"Wiley","issue":"46","license":[{"start":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T00:00:00Z","timestamp":1782604800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T00:00:00Z","timestamp":1782604800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["62175252,62205366,82227806"],"award-info":[{"award-number":["62175252,62205366,82227806"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Single\u2010molecule detection enables precise biomarker quantification, yet conventional endpoint assays suffer from long incubation and nonspecific binding. Kinetic assays resolve these via real\u2010time monitoring of molecular interactions, but their reliance on complex optics has restricted their practical adoption, especially in point\u2010of\u2010care settings where speed, portability, and ease of use are paramount. We present a microlens\u2010assisted platform that enables real\u2010time tracking of single\u2010binding events under conventional bright\u2010field microscopy. An on\u2010chip microlens array amplifies scattering from gold nanoparticle labels, enabling kinetic monitoring with low\u2010magnification optics. Dynamic fingerprints of individual events distinguish specific from nonspecific interactions, achieving wash\u2010free digital biosensing with high sensitivity and specificity. This approach not only preserves the analytical advantages of kinetic profiling but also enables an exceptionally compact and portable optical configuration. Using cardiac troponin I, a time\u2010critical biomarker for acute myocardial infarction, we demonstrate an ultralow detection limit of 0.051 pg mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    and validate clinical applicability in undiluted serum samples, paving the way for miniaturized, high\u2010performance biosensors that can deliver laboratory\u2010grade diagnostics at the point of need.\n                  <\/jats:p>","DOI":"10.1002\/advs.76076","type":"journal-article","created":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T12:07:15Z","timestamp":1782648435000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dynamic Single\u2010Binding Event Profiling With on\u2010Chip Microlenses for Wash\u2010Free Digital Biosensing"],"prefix":"10.1002","volume":"13","author":[{"given":"Tingting","family":"Zhan","sequence":"first","affiliation":[{"name":"Research Center For Biosensing and Intelligence Institute of Biomedical and Health Engineering Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences  Shenzhen China"},{"name":"School of Medicine and Health Key Laboratory of Microsystems and Microstructures Manufacturing (Ministry of Education) Harbin Institute of Technology  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lianyu","family":"Lu","sequence":"additional","affiliation":[{"name":"Research Center For Biosensing and Intelligence Institute of Biomedical and Health Engineering Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9853-5799","authenticated-orcid":false,"given":"Guoqiang","family":"Gu","sequence":"additional","affiliation":[{"name":"Research Center For Biosensing and Intelligence Institute of Biomedical and Health Engineering Shenzhen Institutes of Advanced Technology Chinese 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