{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T09:04:19Z","timestamp":1789376659744,"version":"build-2803163510"},"reference-count":42,"publisher":"Wiley","issue":"51","license":[{"start":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T00:00:00Z","timestamp":1781740800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T00:00:00Z","timestamp":1781740800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["3267190716"],"award-info":[{"award-number":["3267190716"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32072901"],"award-info":[{"award-number":["32072901"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Japanese encephalitis virus (JEV) is a neurotropic flavivirus that causes a substantial threat to human health and livestock; however, the epitranscriptomic mechanisms that support its replication remain poorly defined. Here, we identify a proviral host factor C\n                    <jats:sub>2<\/jats:sub>\n                    H\n                    <jats:sub>2<\/jats:sub>\n                    zinc\u2010finger protein ZNF33B that promotes JEV infection through coupling N\n                    <jats:sup>6<\/jats:sup>\n                    \u2010methyladenosine (m\n                    <jats:sup>6<\/jats:sup>\n                    A) RNA modification to autophagy regulation. Mechanistically, ZNF33B recruits METTL14 to stabilize the METTL3\u2010METTL14 methyltransferase complex, thereby increasing global m\n                    <jats:sup>6<\/jats:sup>\n                    A deposition. Multi\u2010omics analyses reveal that ZNF33B selectively binds m\n                    <jats:sup>6<\/jats:sup>\n                    A\u2010modified sites within the antiviral transcript\n                    <jats:italic>Trim25<\/jats:italic>\n                    (c.1567 and c.1669\u00a0bp) to accelerate its decay. We further demonstrate that TRIM25 functions as an E3 ubiquitin ligase that catalyzes K48\u2010linked ubiquitination of ATG7 at lysines 389 and 423, leading to its proteasomal degradation and ultimately suppressing autophagic flux. In contrast, ZNF33B\u2010mediated\n                    <jats:italic>Trim25<\/jats:italic>\n                    degradation counteracts its inhibitory effect on autophagy, creating a favorable environment for viral replication. In vivo, adeno\u2010associated virus (AAV)\u2010mediated ZNF33B delivery increases mouse brain m\n                    <jats:sup>6<\/jats:sup>\n                    A levels, decreases TRIM25 expression, elevates ATG7 abundance, exacerbates JEV\u2010induced neuropathology, and accelerates mouse mortality. Together, these findings reveal a previously uncharacterized ZNF33B\u2010m\n                    <jats:sup>6<\/jats:sup>\n                    A\u2010TRIM25\u2010autophagy axis that JEV hijacks to evade host antiviral responses, providing new insights into flaviviral pathogenesis and potential therapeutic targets.\n                  <\/jats:p>","DOI":"10.1002\/advs.76122","type":"journal-article","created":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T19:33:05Z","timestamp":1781811185000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["ZNF33B Promotes Japanese Encephalitis Virus Infection by Regulating the Stability of M\n                    <sup>6<\/sup>\n                    A\u2010Modified\n                    <i>Trim25<\/i>\n                    to Control the Autophagy Process"],"prefix":"10.1002","volume":"13","author":[{"given":"Jian","family":"Du","sequence":"first","affiliation":[{"name":"National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan 430070 P. R. China"},{"name":"College of Veterinary Medicine Huazhong Agricultural University Wuhan 430070 P. R. China"},{"name":"The Cooperative Innovation Centre for Sustainable Pig Production Huazhong Agricultural University Wuhan 430070 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunwei","family":"Li","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan 430070 P. R. China"},{"name":"College of Veterinary Medicine Huazhong Agricultural University Wuhan 430070 P. R. China"},{"name":"The Cooperative Innovation Centre for Sustainable Pig Production Huazhong Agricultural University Wuhan 430070 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiyuan","family":"Luo","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan 430070 P. R. China"},{"name":"College of Veterinary Medicine Huazhong Agricultural University Wuhan 430070 P. R. China"},{"name":"The Cooperative Innovation Centre for Sustainable Pig Production Huazhong Agricultural University Wuhan 430070 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huizhi","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan 430070 P. R. China"},{"name":"College of Veterinary Medicine Huazhong Agricultural University Wuhan 430070 P. R. China"},{"name":"The Cooperative Innovation Centre for Sustainable Pig Production Huazhong Agricultural University Wuhan 430070 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyan","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan 430070 P. R. China"},{"name":"College of Veterinary Medicine Huazhong Agricultural University Wuhan 430070 P. R. China"},{"name":"The Cooperative Innovation Centre for Sustainable Pig Production Huazhong Agricultural University Wuhan 430070 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suya","family":"Wang","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan 430070 P. R. China"},{"name":"College of Veterinary Medicine Huazhong Agricultural University Wuhan 430070 P. R. China"},{"name":"The Cooperative Innovation Centre for Sustainable Pig Production Huazhong Agricultural University Wuhan 430070 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huanchun","family":"Chen","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan 430070 P. R. China"},{"name":"College of Veterinary Medicine Huazhong Agricultural University Wuhan 430070 P. R. China"},{"name":"The Cooperative Innovation Centre for Sustainable Pig Production Huazhong Agricultural University Wuhan 430070 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongli","family":"Xu","sequence":"additional","affiliation":[{"name":"Hubei Cancer Hospital, Tongji Medical College, Huazhong University of Science and Technology Hubei Provincial Clinical Research Center For Colorectal Cancer Wuhan 430079 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangmin","family":"Li","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan 430070 P. R. China"},{"name":"College of Veterinary Medicine Huazhong Agricultural University Wuhan 430070 P. R. China"},{"name":"The Cooperative Innovation Centre for Sustainable Pig Production Huazhong Agricultural University Wuhan 430070 P. R. China"},{"name":"Hubei Jiangxia Laboratory Wuhan 430200 P. R. 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