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Here, we systematically evaluated cold tolerance and quantified 12 key tea quality\u2010related metabolites over two consecutive years of 108 geographically diverse tea accessions in China. Multi\u2010trait genome\u2010wide association mapping identified a central hub regulator, COLD AND CATECHINS REGULATOR 1 (CCR1), significantly associated with cold tolerance and catechins biosynthesis in tea plants. Functional analysis demonstrated that\n                    <jats:italic>CsCCR1<\/jats:italic>\n                    positively regulates cold tolerance and catechins accumulation in tea plants, and that a missense variation (A\u2010to\u2010C) in\n                    <jats:italic>CsCCR1<\/jats:italic>\n                    significantly enhances cold tolerance and catechins accumulation. Exogenous application of catechins (EGCG\/EGC\/EC) could also significantly alleviate cold\u2010induced oxidative damage through ROS scavenging. We demonstrated that\n                    <jats:italic>CsCCR1<\/jats:italic>\n                    , activated by upstream regulators CsLUX and CsKUA1, directly activates catechin biosynthetic genes (\n                    <jats:italic>CsCHS1<\/jats:italic>\n                    ,\n                    <jats:italic>CsFLS1<\/jats:italic>\n                    ,\n                    <jats:italic>CsSCPL4<\/jats:italic>\n                    ,\n                    <jats:italic>CsSCPL5<\/jats:italic>\n                    ) and a novel cold\u2010responsive gene\n                    <jats:italic>CsELIP1<\/jats:italic>\n                    . Furthermore, CsCCR1 physically interacts with CsCBF1\/3 to form a transcriptional activation complex that synergistically amplifies expression of these downstream targets, thereby simultaneously modulating cold tolerance and catechins biosynthesis. Our findings underscore the importance of\n                    <jats:italic>CsCCR1<\/jats:italic>\n                    in linking cold tolerance with quality formation of tea plants, offering targets for breeding elite tea cultivars with superior cold resistance and quality.\n                  <\/jats:p>","DOI":"10.1002\/advs.76225","type":"journal-article","created":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T10:56:14Z","timestamp":1782125774000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Natural Variation of COLD and CATECHINS REGULATOR 1 Coordinately Fine\u2010Tunes Cold Tolerance and Tea Quality in Tea Plants"],"prefix":"10.1002","volume":"13","author":[{"given":"Yanli","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization Anhui Agricultural University  Hefei 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and Resource Utilization Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qijuan","family":"Gao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangdong","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6615-1598","authenticated-orcid":false,"given":"Zhaoliang","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization Anhui Agricultural University  Hefei 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