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However, the cellular basis and regulatory programs governing sexual development and egg production are poorly characterized. Here, we constructed a dynamic single\u2010cell atlas of\n                    <jats:italic>Schistosoma japonicum<\/jats:italic>\n                    (\n                    <jats:italic>S. japonicum<\/jats:italic>\n                    ) covering key development stages of sexual maturation and egg production, and integrated it for the first time with spatial transcriptomics to map tissue\u2011resolved cellular niches. Through bioinformatic analysis and experimental validation, we identified several critical cell populations and regulatory networks that drive parasite development and egg laying. By mapping\n                    <jats:italic>de novo<\/jats:italic>\n                    identified transcription factors (TFs) onto these atlases, we generated a comprehensive spatiotemporal expression profile of TFs and define the regulatory programs of several tissue\u2010specific TFs including\n                    <jats:italic>Zfp<\/jats:italic>\n                    ,\n                    <jats:italic>Fbp3<\/jats:italic>\n                    , and\n                    <jats:italic>Lim<\/jats:italic>\n                    . Integrated re\u2011clustering further revealed and validated key regulators of germline stem\u2010cell differentiation in\n                    <jats:italic>S. japonicum<\/jats:italic>\n                    . Finally, comparative analyses with\n                    <jats:italic>S. mansoni<\/jats:italic>\n                    single\u2010cell datasets revealed both conserved and species\u2011divergent cellular plasticity, providing unprecedented insights into their distinct biological traits. Collectively, our comprehensive study establishes a foundational resource for understanding schistosome biology, deciphers regulatory programs underlying cell differentiation and pathogenicity, and accelerates the identification of key molecules involved in sexual development against schistosomiasis.\n                  <\/jats:p>","DOI":"10.1002\/advs.76329","type":"journal-article","created":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T22:21:46Z","timestamp":1783808506000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Single\u2010Cell and Spatial Transcriptomics Unveil Key Regulators Governing Cell Differentiation for\n                    <i>Schistosoma japonicum<\/i>\n                    Sexual Development"],"prefix":"10.1002","volume":"13","author":[{"given":"Zhigang","family":"Lu","sequence":"first","affiliation":[{"name":"Shanghai Tenth People\u2019s Hospital, Institute For Infectious Diseases and Vaccine Development Clinical Center for Brain and Spinal Cord Research, Affiliated Shanghai Blue Cross Brain Hospital, Tongji University School of Medicine  Shanghai China"},{"name":"Faculty of Natural Sciences University of Hohenheim, Stuttgart  Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxu","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Biotechnology Jiangsu University of Science and Technology  Zhen Jiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shun","family":"Li","sequence":"additional","affiliation":[{"name":"Shanghai Veterinary Research Institute Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology of Ministry of Agriculture and Rural Affairs  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuxin","family":"Li","sequence":"additional","affiliation":[{"name":"Shanghai Veterinary Research Institute Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology of Ministry of Agriculture and Rural Affairs  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengfei","family":"Du","sequence":"additional","affiliation":[{"name":"Shanghai Veterinary Research Institute Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology of Ministry of Agriculture and Rural Affairs  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yameng","family":"Zheng","sequence":"additional","affiliation":[{"name":"Shanghai Tenth People\u2019s Hospital, Institute For Infectious Diseases and Vaccine Development Clinical Center for Brain and Spinal Cord Research, Affiliated Shanghai Blue Cross Brain Hospital, Tongji University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Liu","sequence":"additional","affiliation":[{"name":"Shanghai Tenth People\u2019s Hospital, Institute For Infectious Diseases and Vaccine Development Clinical Center for Brain and Spinal Cord Research, Affiliated Shanghai Blue Cross Brain Hospital, Tongji University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanyan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Faculty of Natural Sciences University of Hohenheim, Stuttgart  Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omri","family":"Wurtzel","sequence":"additional","affiliation":[{"name":"The School of Neurobiology, Biochemistry &amp; Biophysics, George S. 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