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This study shows, using viability studies, post\u2010mortem corpus callosum (CC) tissue OSCs cultured with a chemically defined serum\u2010free culturing medium, maintaining viability for at least 8 days in vitro (DIV). The inclusion of CC simplifies myelin\u2010focused research, and its potential is highlighted by visualizing molecular information within myelin by the use of label\u2010free Coherent anti\u2010Stokes Raman spectroscopy (CARS) imaging. This study delineates a window of 32 h after time of death (TOD) wherein myelin remains unaffected from base measurements. Finally, as a proof\u2010of\u2010concept, the CC tissue of a human\u2010specific disease characterized by myelin degeneration, multiple sclerosis (MS), reveals signs of altered myelin spectral profiles when compared to non\u2010MS myelin. 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