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Using correlative cryo\u2010electron tomography in primary cortical neurons and brain tissue from an NIID mouse model, we show that polyG inclusions are built from interconnected ribbon\u2010like assemblies rather than canonical amyloid fibrils. PolyG populates multiple compartment\u2010specific ribbon states, including a nuclear ribbon network enriched in 26S proteasomes and two cytoplasmic ribbon packing states with sharply different proteasome accessibility. In the cytoplasm, ribbon assemblies frequently contact endomembranes\u2014particularly ER\u2010like membranes\u2014and these interactions coincide with membrane deformation, consistent with transcriptomic dysregulation of ER\u2010stress responses\u2010related genes. 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