{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T03:50:28Z","timestamp":1792036228400,"version":"4.4.0"},"reference-count":45,"publisher":"Wiley","issue":"57","license":[{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U23A20460"],"award-info":[{"award-number":["U23A20460"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82273350"],"award-info":[{"award-number":["82273350"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82173061"],"award-info":[{"award-number":["82173061"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82341030"],"award-info":[{"award-number":["82341030"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Noninvasive diagnosis and longitudinal surveillance of urothelial carcinoma (UC) remain clinically challenging. Here, we developed and prospectively validate UCtracker, a urine DNA methylation\u2013based deep learning model for UC detection and postoperative recurrence monitoring. UC\u2010specific differentially methylated regions (DMRs) were identified by high\u2010depth whole\u2010genome bisulfite sequencing of UC tissues, paired adjacent normal tissues, and non\u2010tumor urine samples. UCtracker was constructed using the top 2000 hypomethylated DMRs and a convolutional neural network\u2013bidirectional long short\u2010term memory architecture. In an internal validation cohort (\n                    <jats:italic>n<\/jats:italic>\n                    = 165), UCtracker achieved a sensitivity of 94.6% and a specificity of 94.4%, and maintained high performance in an independent multicenter cohort (\n                    <jats:italic>n<\/jats:italic>\n                    = 55), with a sensitivity of 90.6% and a specificity of 91.3%. UCtracker showed higher sensitivity than UroVysion fluorescence in situ hybridization for T1 tumors, high\u2010grade tumors, and bladder UC. Subsampling analyses demonstrated stable diagnostic performance even at ultralow sequencing depths. In postoperative surveillance, longitudinal urine profiling of 131 samples from 48 UC patients detected 94.1% of recurrence events and identified recurrence up to 250 days before clinical confirmation. These findings support UCtracker as a highly accurate and cost\u2010effective urine\u2010based tool for UC diagnosis, postoperative surveillance, and personalized patient management.\n                  <\/jats:p>","DOI":"10.1002\/advs.76710","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T11:17:33Z","timestamp":1785755853000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["UCtracker: A Deep Learning\u2013Based DNA Methylation Model for Noninvasive Diagnosis and Recurrence Surveillance of Urothelial Carcinoma in a Prospective Study"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4885-899X","authenticated-orcid":false,"given":"Shengwei","family":"Xiong","sequence":"first","affiliation":[{"name":"Department of Urology Institute of Urology Beijing Key Laboratory of Precision Medicine and Innovative Translation for Urogenital Diseases Peking University First Hospital National Urological Cancer Center Peking University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaojie","family":"Li","sequence":"additional","affiliation":[{"name":"China National Center for Bioinformation Beijing Institute of Genomics Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0087-9548","authenticated-orcid":false,"given":"Yucai","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Urology Institute of Urology Beijing Key Laboratory of Precision Medicine and Innovative Translation for Urogenital Diseases Peking University First Hospital National Urological Cancer Center Peking University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Liang","sequence":"additional","affiliation":[{"name":"China National Center for Bioinformation Beijing Institute of Genomics Chinese Academy of Sciences  Beijing China"},{"name":"Institute for Regenerative Biology and Medicine Chinese Institutes for Medical Research  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heng","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Urology Institute of Urology Beijing Key Laboratory of Precision Medicine and Innovative Translation for Urogenital Diseases Peking University First Hospital National Urological Cancer Center Peking University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Urology Institute of Urology Beijing Key Laboratory of Precision Medicine and Innovative Translation for Urogenital Diseases Peking University First Hospital National Urological Cancer Center Peking University  Beijing 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Urology Institute of Urology Beijing Key Laboratory of Precision Medicine and Innovative Translation for Urogenital Diseases Peking University First Hospital National Urological Cancer Center Peking University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihua","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Urology Institute of Urology Beijing Key Laboratory of Precision Medicine and Innovative Translation for Urogenital Diseases Peking University First Hospital National Urological Cancer Center Peking University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0410-6489","authenticated-orcid":false,"given":"Yanqing","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of Urology 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