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Boiling histotripsy (BH), a mechanical form of focused ultrasound, allows for non\u2010invasive and non\u2010ionizing tissue destruction. As mechanically fractionating tumors with BH may potentiate T cell\u2010mediated anti\u2010tumor responses, we addressed gaps in knowledge regarding the impact BH has on antigen\u2010presenting cell (APC) presence in the tumor microenvironment (TME), antigen acquisition, and conventional dendritic cell (cDC) activation states in settings where BH does or does not induce a primary tumor growth response. When BH treatment immediately inhibited melanoma outgrowth, APC presence in the TME was significantly reduced, tumor antigen acquisition amongst APCs remained elevated when compared to controls, and intratumoral immune activation was observed distinctly in the cDC1 subset. Cavitation scoring based on real\u2010time B\u2010mode imaging signatures characteristic of BH\u2010induced tissue destruction demonstrated that BH\u2010mediated destruction strongly correlates with tumor antigen presence in the draining lymph nodes 24\u00a0h post\u2010ablation. Collectively, these findings reveal that immediate BH\u2010driven tumor growth control in a melanoma model stratifies distinct intratumoral immune states; positioning early growth response as a biologically informative prognostic indicator of BH\u2010induced intratumoral immune activation.<\/jats:p>","DOI":"10.1002\/advs.76722","type":"journal-article","created":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T12:15:33Z","timestamp":1784895333000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Immediate Growth Control in Response to Boiling Histotripsy is Prognostic for Intratumoral Immune Activation"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9167-1285","authenticated-orcid":false,"given":"Lydia E.","family":"Kitelinger","sequence":"first","affiliation":[{"name":"Department of Pathology University of Virginia  Charlottesville Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew R.","family":"DeWitt","sequence":"additional","affiliation":[{"name":"Department of Radiology &amp; Medical Imaging, Department of Biomedical Engineering University of Virginia  Charlottesville Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-2091-5762","authenticated-orcid":false,"given":"Carly M.","family":"Van Wagoner","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Cancer Biology University of Virginia  Charlottesville Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire A.","family":"Conarroe","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Virginia  Charlottesville Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles C.","family":"Funk","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Virginia  Charlottesville Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-7728-6553","authenticated-orcid":false,"given":"Aaron B.","family":"Streit","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Virginia  Charlottesville Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"AeRyon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Virginia  Charlottesville Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0237-2102","authenticated-orcid":false,"given":"Richard J.","family":"Price","sequence":"additional","affiliation":[{"name":"Department of Radiology &amp; Medical Imaging, Department of Biomedical Engineering University of Virginia  Charlottesville Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6141-3261","authenticated-orcid":false,"given":"Timothy N. 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