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Here we develop Super\u2010multiplexed Label\u2010free Raman Imaging (SLRI) to generate 2D\/3D biochemical maps of intact\n                    <jats:italic>Drosophila<\/jats:italic>\n                    testes. SLRI delineates major testicular cell types and provides the first in situ, stage\u2010resolved mapping of cytoplasmic RBs at two discrete phases. We further identify a differentiation\u2010arrest\u2010associated metabolic trajectory in spermatogonial clusters, characterized by coordinated spatiotemporal decay of specific biochemical species. Across 42 spatiotemporally variant metabolites and metabolic pathways, SLRI reveals RB\u2010 and arrest\u2010enriched signatures. Notably, RBs and arrested spermatogonia display significant biochemical overlap, alongside unique spectral signatures. Furthermore, we uncovered a ring\u2010like, punctate arrangement of lipids, amino acids, and vitamins encircling RBs, suggesting structured metabolite partitioning during cytoplasmic remodeling. Collectively, SLRI establishes a general framework for high\u2010dimensional metabolic mapping and remodeling analysis in intact tissues.\n                  <\/jats:p>","DOI":"10.1002\/advs.76757","type":"journal-article","created":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T12:18:25Z","timestamp":1784636305000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Super\u2010Multiplexed Label\u2010Free Raman Imaging Uncovers Novel Testicular Metabolic Couplings for Residual Body and Spermatogonial Differentiation"],"prefix":"10.1002","volume":"13","author":[{"given":"Jiaxin","family":"Li","sequence":"first","affiliation":[{"name":"Clinical Research Center for Reproductive Genetics Center for Reproductive Medicine Affiliated Maternity and Child Health Care Hospital of Nantong University Medical School of Nantong University Nantong China"},{"name":"Institute of Reproductive Medicine Medical School Nantong 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Nanjing Medical University Suzhou Municipal Hospital Gusu School Nanjing Medical University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyu","family":"Tang\u2010Yang","sequence":"additional","affiliation":[{"name":"Institute of Reproductive Medicine Medical School Nantong University  Nantong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanzhi","family":"Zang","sequence":"additional","affiliation":[{"name":"Institute of Reproductive Medicine Medical School Nantong University  Nantong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuwen","family":"Du","sequence":"additional","affiliation":[{"name":"Institute of Reproductive Medicine Medical School Nantong University  Nantong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anran","family":"Dong","sequence":"additional","affiliation":[{"name":"Institute of Reproductive Medicine Medical School Nantong University  Nantong 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