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Here, a robust strategy was presented to develop potent cancer vaccines toward TAMs via a multi\u2010functional adjuvant, breaking through the conventional vaccine design centered on dendritic cells (DCs). To address this issue, rigidity\u2010tunable nanovesicles (P\u2010P\n                    <jats:sub>m<\/jats:sub>\n                    ) were engineered by surface decoration of Chol\u2010PItEG\n                    <jats:sub>m<\/jats:sub>\n                    with precise regulation of chain lengths. The P\u2010P\n                    <jats:sub>m<\/jats:sub>\n                    nanovesicle exerted mechanically induced adjuvanticity through not only enhancing endocytosis in a rigidity\u2010dependent manner to facilitate antigen delivery and processing in TAMs, but also activating the mechanosensitive Piezo1\/YAP\/TAZ signaling pathway to promote M2\u2010like TAMs reprogramming. Furthermore, the rigid P\u2010P\n                    <jats:sub>m<\/jats:sub>\n                    nanovesicle could efficiently co\u2010encapsulate antigenic gp100 peptide and toll\u2010like receptor agonist resiquimod (R848) to form an anti\u2010melanoma vaccine P@Rg\u2010P\n                    <jats:sub>m<\/jats:sub>\n                    . After intravenously injected, CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cell infiltration was increased while immune\u2010suppressive cell level of myeloid derived suppressor cells (MDSCs) and regulatory T cells (Tregs) were decreased along with elevated levels of proinflammatory factors including IL\u20101\u03b2, IFN\u2010\u03b3, and IL\u201012, demonstrating the effective reversal of immunosuppressive TME. Consequently, P@Rg\u2010P\n                    <jats:sub>m<\/jats:sub>\n                    demonstrates significant antitumor efficacy against B16\u2010F10 melanoma in both therapeutic and prophylactic models without any assistance of other therapies.\n                  <\/jats:p>","DOI":"10.1002\/advs.76773","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T12:10:20Z","timestamp":1785154220000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Nanovesicles With Mechanically Induced Adjuvanticity for Robust Melanoma Vaccination Toward Tumor\u2010Associated Macrophages"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1923-6892","authenticated-orcid":false,"given":"Bangyue","family":"Luo","sequence":"first","affiliation":[{"name":"Department of Polymer Science and Engineering Ministry of Educational (MOE) Key Laboratory of Macromolecular 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