{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T12:45:29Z","timestamp":1785761129538,"version":"3.56.0"},"reference-count":76,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["3278\/2\u20101"],"award-info":[{"award-number":["3278\/2\u20101"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    B cell activation typically involves binding of presented antigen by the B cell receptor. Ensuing intracellular signaling with Ca\n                    <jats:sup>2+<\/jats:sup>\n                    mobilization, cytoskeletal remodeling, and transcriptional changes culminates in an appropriate B cell response. Recently, our group developed an antigen receptor\u2010independent, purely mechanobiological activation platform for T cells. Nanotopological stimulation by exposure to a porous membrane, resulted in robust T cell activation. Whether and how B cells are activated by this substrate on a mechanistic level is presently unknown. Here we report that nanoporous stimulation indeed results in B cell activation, where B cells extended nanopore penetrating microvilli and exhibited activation hallmarks including intracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    spikes, phosphorylation of signaling components, and cell surface expression of the activation marker cluster of differentiation (CD) 69, thus recapitulating T cell behavior. This is at least partially mediated by mechanosensitive cation channels, as determined by pharmacology. In summary, our findings demonstrate that B cells are amenable to antigen receptor\u2010independent activation via nanopores, expanding our knowledge on B cell activation mechanisms.\n                  <\/jats:p>","DOI":"10.1002\/advs.76869","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T11:49:28Z","timestamp":1785757768000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["B Cells are Activated via a Nanoporous Interface that Stabilizes Microvilli and Engages Mechanosensitive Ion Channels"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6096-1853","authenticated-orcid":false,"given":"Nozie D.","family":"Aghaizu","sequence":"first","affiliation":[{"name":"Institute for Biology Experimental Biophysics\/Mechanobiology Humboldt Universit\u00e4t Zu Berlin  Berlin Germany"},{"name":"Experimental and Clinical Research Center (ECRC) at Charit\u00e9 Universit\u00e4tsmedizin Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willi","family":"Weber","sequence":"additional","affiliation":[{"name":"Institute for Biology Experimental Biophysics\/Mechanobiology Humboldt Universit\u00e4t Zu Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2207-177X","authenticated-orcid":false,"given":"Nadine","family":"Strempel","sequence":"additional","affiliation":[{"name":"Charit\u00e9 Universit\u00e4tsmedizin  Berlin Germany"},{"name":"German Center for Neurodegenerative Diseases (DZNE)  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9503-3177","authenticated-orcid":false,"given":"Asya","family":"Polat","sequence":"additional","affiliation":[{"name":"Institute for Biology Experimental Biophysics\/Mechanobiology Humboldt Universit\u00e4t Zu Berlin  Berlin 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